Takano Toru, Miyauchi Akira, Matsuzuka Fumio, Yoshida Hiroshi, Notomi Tsugunori, Kuma Kanji, Amino Nobuyuki
Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
J Clin Endocrinol Metab. 2005 Feb;90(2):720-3. doi: 10.1210/jc.2004-0951. Epub 2004 Nov 9.
Distinguishing between thyroid malignant lymphoma and lymphocytic thyroiditis (Hashimoto's thyroiditis) is quite difficult and problematic. B cell lymphomas display clonal Ig heavy-chain (IgH) gene rearrangement, and Southern blot hybridization (SBH) is often used for detection of the monoclonality of the IgH gene. However, SBH is often problematic because it requires a large volume of samples. We examined the efficiency in detecting the monoclonality of IgH gene in thyroid malignant lymphomas by vectorette PCR, which we started with only 200 ng of genomic DNA. Monoclonality was detected in 36 of 47 (76.6%) malignant lymphomas, whereas it was not detected in 10 samples of tissue of Hashimoto's thyroiditis. The sensitivity was almost the same as that with SBH in which monoclonality was detected in 33 of 45 (73.3%) malignant lymphomas. These results suggest that vectorette PCR may be a substitute for SBH, and because it requires only a small volume of samples, it may be used in the analysis of aspiration biopsies.
区分甲状腺恶性淋巴瘤和淋巴细胞性甲状腺炎（桥本甲状腺炎）非常困难且存在问题。B细胞淋巴瘤表现出克隆性Ig重链（IgH）基因重排，Southern印迹杂交（SBH）常被用于检测IgH基因的单克隆性。然而，SBH常常存在问题，因为它需要大量样本。我们通过载体PCR检测了甲状腺恶性淋巴瘤中IgH基因单克隆性的检测效率，该方法仅从200 ng基因组DNA开始。在47例恶性淋巴瘤中有36例（76.6%）检测到单克隆性，而在10例桥本甲状腺炎组织样本中未检测到。其敏感性与SBH几乎相同，在45例恶性淋巴瘤中有33例（73.3%）检测到单克隆性。这些结果表明载体PCR可能是SBH的替代方法，并且由于它只需要少量样本，可用于细针穿刺活检的分析。
