Savant D V, Ranade D R
Division of Microbial Sciences, Agharkar Research Institute, Pune 411 004, India.
Water Sci Technol. 2004;50(6):109-14.
To operate anaerobic digesters successfully under acidic conditions, hydrogen utilizing methanogens which can grow efficiently at low pH and tolerate high volatile fatty acids (VFA) are desirable. An acid tolerant hydrogenotrophic methanogen viz. Methanobrevibacter acididurans isolated from slurry of an anaerobic digester running on alcohol distillery wastewater has been described earlier by this lab. This organism could grow optimally at pH 6.0. In the experiments reported herein, M. acididurans showed better methanogenesis under acidic conditions with high VFA, particularly acetate, than Methanobacterium bryantii, a common hydrogenotrophic inhabitant of anaerobic digesters. Addition of M. acididurans culture to digesting slurry of acidogenic as well as methanogenic digesters running on distillery wastewater showed increase in methane production and decrease in accumulation of volatile fatty acids. The results proved the feasibility of application of M. acididurans in anaerobic digesters.
为了在酸性条件下成功运行厌氧消化器，需要能够在低pH值下高效生长并耐受高挥发性脂肪酸（VFA）的利用氢气的产甲烷菌。本实验室之前已经描述过一种耐酸的氢营养型产甲烷菌，即从以酒精厂废水为原料运行的厌氧消化器的污泥中分离出的嗜酸甲烷短杆菌。这种微生物在pH 6.0时生长最佳。在本文报道的实验中，与厌氧消化器中常见的氢营养型嗜热栖热甲烷杆菌相比，嗜酸甲烷短杆菌在含有高VFA（特别是乙酸盐）的酸性条件下表现出更好的产甲烷作用。将嗜酸甲烷短杆菌培养物添加到以酒厂废水为原料运行的产酸型和产甲烷型消化器的消化污泥中，结果表明甲烷产量增加，挥发性脂肪酸的积累减少。结果证明了嗜酸甲烷短杆菌应用于厌氧消化器的可行性。
