Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland.
Molecules. 2019 Jul 18;24(14):2624. doi: 10.3390/molecules24142624.
In this study, we used a multifaceted approach to select robust bioaugmentation candidates for enhancing biogas production and to demonstrate the usefulness of a genome-centric approach for strain selection for specific bioaugmentation purposes. We also investigated the influence of the isolation source of bacterial strains on their metabolic potential and their efficiency in enhancing anaerobic digestion. Whole genome sequencing, metabolic pathway reconstruction, and physiological analyses, including phenomics, of phylogenetically diverse strains, sp. POC4, sp. POC9 (both isolated from sewage sludge) and sp. LPMIX5 (isolated from an agricultural biogas plant) showed their diverse enzymatic activities, metabolic versatility and ability to survive under varied growth conditions. All tested strains display proteolytic, lipolytic, cellulolytic, amylolytic, and xylanolytic activities and are able to utilize a wide array of single carbon and energy sources, as well as more complex industrial by-products, such as dairy waste and molasses. The specific enzymatic activity expressed by the three strains studied was related to the type of substrate present in the original isolation source. Bioaugmentation with sewage sludge isolates-POC4 and POC9-was more effective for enhancing biogas production from sewage sludge (22% and 28%, respectively) than an approach based on LPMIX5 strain (biogas production boosted by 7%) that had been isolated from an agricultural biogas plant, where other type of substrate is used.
在本研究中，我们采用了多方面的方法来选择稳健的生物强化候选物，以提高沼气产量，并展示基于基因组的方法在针对特定生物强化目的选择菌株方面的有用性。我们还研究了细菌菌株的分离源对其代谢潜力及其在增强厌氧消化中的效率的影响。对具有不同系统发育的菌株（POC4 种、POC9 种[均从污水污泥中分离]和 LPMIX5 种[从农业沼气厂中分离]）进行全基因组测序、代谢途径重建和生理分析（包括表型分析），表明它们具有多样化的酶活性、代谢多功能性和在不同生长条件下生存的能力。所有测试的菌株都显示出蛋白水解、脂肪水解、纤维素分解、淀粉水解和木聚糖水解活性，并且能够利用广泛的单碳和能源来源，以及更复杂的工业副产品，如奶制品废物和糖蜜。这三种研究菌株表达的特定酶活性与原始分离源中存在的底物类型有关。与从农业沼气厂分离的 LPMIX5 菌株（沼气产量提高了 7%）相比，用污水污泥分离物-POC4 和 POC9 进行生物强化更有效地提高了污水污泥的沼气产量（分别提高了 22%和 28%），因为农业沼气厂中使用了其他类型的底物。
