Li S J, Hu J, Liu B L
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, P.R. China.
Methods Find Exp Clin Pharmacol. 2004 Sep;26(7):507-13. doi: 10.1358/mf.2004.26.7.863732.
Micro-sized acrolein-modified PMMA (poly[methyl methacrylate]) microspheres were used as the support for enzyme immobilization. The properties of immobilized enzyme as well as the immobilizing behavior were studied. The immobilized amount reached 76.8 mg.g(-1) support, which is relatively high compared with some analogous supports. The K(m) value was larger and the V(max) was smaller in the immobilized form than in the free form. Better resistance of the immobilized enzyme against the temperature, time and pH changes showed that the modified PMMA microspheres could be a good support for immobilization of pepsin.
微米级丙烯醛改性聚甲基丙烯酸甲酯（PMMA）微球被用作酶固定化的载体。研究了固定化酶的性质以及固定化行为。固定化量达到76.8 mg·g⁻¹载体，与一些类似载体相比相对较高。固定化形式的K(m)值较大，V(max)较小。固定化酶对温度、时间和pH变化具有更好的抗性，表明改性PMMA微球可能是固定化胃蛋白酶的良好载体。
