Shin J-H, Delabar J-M, Lubec G
Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
Amino Acids. 2004 Dec;27(3-4):339-44. doi: 10.1007/s00726-004-0131-3. Epub 2004 Nov 5.
Phosphoprotein enriched in astrocytes of 15 kDa (PEA-15) is a small protein that was first identified as an abundant phosphoprotein in brain. PEA-15 was characterised so far at the immunochemical level and by a microsequencing attempt. In order to update characterisation of this important structure by advanced methodology unambiguously identifying proteins independent of antibody availability and specificity, we used a proteomic method for this purpose: Performing protein profiling in mouse hippocampi using two dimensional gel electrophoresis with subsequent mass spectrometrical (MS/MS) identification we detected this protein and demonstrate proteomic characterisation of PEA-15 (Q62048). This study enables further specific and unambiguous determination serving as an analytical tool.
15 kDa星形胶质细胞富集磷蛋白（PEA - 15）是一种小蛋白，最初被鉴定为大脑中一种丰富的磷蛋白。到目前为止，PEA - 15已在免疫化学水平以及通过微量测序尝试进行了表征。为了通过先进方法明确鉴定与抗体可用性和特异性无关的蛋白质来更新对这一重要结构的表征，我们为此使用了一种蛋白质组学方法：在小鼠海马体中使用二维凝胶电泳进行蛋白质谱分析，随后进行质谱（MS/MS）鉴定，我们检测到了这种蛋白质，并展示了PEA - 15（Q62048）的蛋白质组学表征。这项研究能够进行进一步的特异性和明确的测定，作为一种分析工具。
