Maes Piet, Van Doren Els, Denys Barbara, Thoelen Inge, Rahman Mustafizur, Vijgen Leen, Van Ranst Marc
Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Belgium.
Biochem Biophys Res Commun. 2004 Dec 17;325(3):711-5. doi: 10.1016/j.bbrc.2004.10.084.
To achieve the goal of poliovirus eradication, surveillance of endemic areas is a crucial step in the poliovirus eradication program. Currently, six countries still have endemic poliovirus. We have tested a novel method which uses SDS/EDTA-treated chromatography paper strips to collect and transport poliovirus-containing stool samples. The SDS/EDTA-treated paper strips were soaked with different dilutions of poliovirus-containing feces and stored at different temperatures. After storing the SDS/EDTA paper strips for 5 months at 37 degrees C, poliovirus RNA could be successfully amplified using RT-PCR. Infectivity of wild-type poliovirus type 1, 2, and 3 was lost upon contact with the SDS/EDTA-treated strips. This easy, inexpensive, and biosafe chromatography paper strip method for the collection and transportation of poliovirus samples can be of use in poliovirus surveillance and polio vaccination programs.
为实现根除脊髓灰质炎病毒的目标，对流行地区进行监测是脊髓灰质炎病毒根除计划中的关键一步。目前，仍有六个国家存在脊髓灰质炎病毒地方性流行。我们测试了一种新方法，该方法使用经十二烷基硫酸钠/乙二胺四乙酸（SDS/EDTA）处理的层析纸条来收集和运输含脊髓灰质炎病毒的粪便样本。将经SDS/EDTA处理的纸条浸泡在不同稀释度的含脊髓灰质炎病毒粪便中，并在不同温度下储存。在37摄氏度下将SDS/EDTA纸条储存5个月后，可使用逆转录聚合酶链反应（RT-PCR）成功扩增脊髓灰质炎病毒RNA。1型、2型和3型野生型脊髓灰质炎病毒与经SDS/EDTA处理的纸条接触后失去感染性。这种用于收集和运输脊髓灰质炎病毒样本的简便、廉价且生物安全的层析纸条方法可用于脊髓灰质炎病毒监测和脊髓灰质炎疫苗接种计划。
Zotero 插件