用于粪便样本中轮状病毒RNA收集、运输和储存的层析纸条法。
Chromatography paper strip method for collection, transportation, and storage of rotavirus RNA in stool samples.
作者信息
Rahman Mustafizur, Goegebuer Truus, De Leener Karolien, Maes Piet, Matthijnssens Jelle, Podder Goutam, Azim Tasnim, Van Ranst Marc
机构信息
Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Belgium.
出版信息
J Clin Microbiol. 2004 Apr;42(4):1605-8. doi: 10.1128/JCM.42.4.1605-1608.2004.
Abstract
We developed a novel method that uses sodium dodecyl sulfate-EDTA-treated chromatography paper strips to collect unconcentrated fresh stool samples. After the paper strips were stored for 4 months at room temperature, rotavirus RNA could be successfully amplified by using reverse transcriptase PCR. The use of filter paper strips as a specimen support allows (self-)collection of stool samples by untrained persons. Diarrheal stool samples from remote areas can be stored and transported to a central diagnostic laboratory without the need for freezers or special shipping conditions. This convenient and inexpensive rotavirus sample collection system can be of use in epidemiological surveillance studies and vaccine trials.
摘要
我们开发了一种新方法，该方法使用经十二烷基硫酸钠-乙二胺四乙酸处理的层析纸条来收集未经浓缩的新鲜粪便样本。将纸条在室温下保存4个月后，可通过逆转录聚合酶链反应成功扩增轮状病毒RNA。使用滤纸作为样本载体，未经培训的人员也可（自行）采集粪便样本。偏远地区的腹泻粪便样本无需冷冻设备或特殊运输条件即可储存并运送至中央诊断实验室。这种方便且经济的轮状病毒样本采集系统可用于流行病学监测研究和疫苗试验。
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