酿酒酵母的产酸突变体。

Acid excreting mutants of yeast Saccharomyces cerevisiae.

作者信息

Machnicka B, Grochowalska R, Boniewska-Bernacka E, Słomińska L, Lachowicz T M

机构信息

Institute of Biotechnology and Environmental Protection, University of Zielona Góra, Monte Cassino 21b, 65-001 Zielona Góra, Poland.

出版信息

Biochem Biophys Res Commun. 2004 Dec 17;325(3):1030-6. doi: 10.1016/j.bbrc.2004.10.130.

DOI:10.1016/j.bbrc.2004.10.130

PMID:15541392

Abstract

Saccharomyces cerevisiae mutants acidifying glucose medium containing bromocresol purple were shown to excrete protons when placed in unbuffered water in the absence of any external carbon source. The mutants belong to 16 different complementation groups. Most of them do not grow on glycerol and the excreted protons are associated to particular sets of organic anions such as citrate, aconitate, succinate, fumarate or malate. These novel types of respiratory mutations seem to be located in genes operating in the Krebs or glyoxylate cycle.

摘要

含有溴甲酚紫的葡萄糖培养基酸化的酿酒酵母突变体，在没有任何外部碳源的情况下置于无缓冲水中时，被证明会分泌质子。这些突变体属于16个不同的互补群。它们中的大多数不能在甘油上生长，并且分泌的质子与特定的有机阴离子组有关，例如柠檬酸盐、乌头酸盐、琥珀酸盐、富马酸盐或苹果酸盐。这些新型的呼吸突变似乎位于参与三羧酸循环或乙醛酸循环的基因中。