Oblak Ewa, Piecuch Agata, Maciaszczyk-Dziubinska Ewa, Wawrzycka Donata
Institute of Genetics and Microbiology, and Institute of Experimental Biology University of Wroclaw, Wroclaw, Poland,
J Biosci. 2016 Dec;41(4):601-614. doi: 10.1007/s12038-016-9644-7.
We investigated the influence of the quaternary ammonium salt (QAS) called IM (N-(dodecyloxycarboxymethyl)- N,N,N-trimethyl ammonium chloride) on yeast cells of the parental strain and the IM-resistant mutant (EO25 IMR) growth. The phenotype of this mutant was pleiotropic. The IMR mutant exhibited resistance to ethanol, osmotic shock and oxidative stress, as well as increased sensitivity to UV. Moreover, it was noted that mutant EO25 appears to have an increased resistance to clotrimazole, ketoconazole, fluconazole, nystatin and cycloheximide. It also tolerated growth in the presence of crystal violet, DTT and metals (selenium, tin, arsenic). It was shown that the presence of IM decreased ergosterol level in mutant plasma membrane and increased its unsaturation. These results indicate changes in the cell lipid composition. Western blot analysis showed the induction of Pma1 level by IM. RT-PCR revealed an increased PMA1 expression after IM treatment.
我们研究了名为IM（N-（十二烷氧基羧甲基）-N，N，N-三甲基氯化铵）的季铵盐对亲本菌株和IM抗性突变体（EO25 IMR）酵母细胞生长的影响。该突变体的表型是多效性的。IMR突变体表现出对乙醇、渗透休克和氧化应激的抗性，以及对紫外线的敏感性增加。此外，还注意到突变体EO25似乎对克霉唑、酮康唑、氟康唑、制霉菌素和环己酰亚胺具有增强的抗性。它还能耐受在结晶紫、二硫苏糖醇和金属（硒、锡、砷）存在下的生长。结果表明，IM的存在降低了突变体质膜中麦角固醇的水平并增加了其不饱和度。这些结果表明细胞脂质组成发生了变化。蛋白质免疫印迹分析显示IM诱导了Pma1水平。逆转录聚合酶链反应显示IM处理后PMA1表达增加。
