Süsleyici Duman Belgin, Oztürk Melek, Yilmazer Selma, Cağatay Penbe, Hatemi Hüsrev
Kadir Has University, Faculty of Medicine, Medical Biology and Genetics Department, Turkey.
Cell Biochem Funct. 2005 Nov-Dec;23(6):399-404. doi: 10.1002/cbf.1162.
We studied the effect of variation at the lipoprotein lipase (LPL) gene locus on the susceptibility of individuals with non-insulin dependent diabetes mellitus (NIDDM) in a population of 110 NIDDM patients and 91 controls. Our objective was to study the relationship between the LPL-Pvu II polymorphism and NIDDM and lipid metabolism. PCR-RFLP was used to determine the DNA polymorphism of the sixth intron of the LPL gene. The frequencies of the genotypes in case and control groups were 29.1 and 30.8% for P+/P+; 45.5 and 36.3% for P+/P-; 25.5 and 33% for P-/P- respectively. There was no significant difference in frequencies of genotypes between the two groups. Logistic regression analysis revealed that triacylglycerol (TAG) and apolipoprotein E levels were associated with NIDDM, whereas Pvu II genotypes were not found as independent risk factors for the disease. Overall this study demonstrates the role of the Pvu II polymorphism in the LPL gene in modulating plasma lipid/lipoprotein levels in patients with NIDDM.
我们在110名非胰岛素依赖型糖尿病（NIDDM）患者和91名对照人群中，研究了脂蛋白脂肪酶（LPL）基因位点变异对NIDDM个体易感性的影响。我们的目的是研究LPL - Pvu II多态性与NIDDM及脂质代谢之间的关系。采用聚合酶链反应 - 限制性片段长度多态性分析（PCR - RFLP）来确定LPL基因第六内含子的DNA多态性。病例组和对照组中基因型的频率分别为：P + / P +型为29.1%和30.8%；P + / P -型为45.5%和36.3%；P - / P -型为25.5%和33%。两组间基因型频率无显著差异。逻辑回归分析显示，甘油三酯（TAG）和载脂蛋白E水平与NIDDM相关，而未发现Pvu II基因型是该疾病的独立危险因素。总体而言，本研究证明了LPL基因中Pvu II多态性在调节NIDDM患者血浆脂质/脂蛋白水平中的作用。
