Li Famei, Zhang Dandan, Lu Xiumei, Wang Yuling, Xiong Zhili
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
Biomed Chromatogr. 2004 Dec;18(10):866-71. doi: 10.1002/bmc.440.
The chiral separation of simendan enantiomers using capillary electrophoresis was studied with beta-cyclodextrin (beta-CD) as chiral selector. The influences of the concentration and pH of borate buffer solution, beta-CD concentration and methanol content in the background electrolyte were investigated. These factors were compared with those in an HPLC with beta-CD as chiral mobile phase additive (CMPA-HPLC). The quantification properties of the developed CE method were examined. A baseline separation of simendan enantiomers was achieved in the background electrolyte of 20 mmol/L borate buffer (pH 11.0) containing 12 mmol/L beta-CD-methanol (50:50 in volume ratio). The CE method is comparable with CMPA-HPLC in chiral resolution, although the optimal pH in CE (11.0) is much higher than that (6.0) in CMPA-HPLC. This chiral CE method is applicable to the quantitative ananlysis and enantiomeric excess value determination of L-simendan.
以β-环糊精（β-CD）作为手性选择剂，研究了采用毛细管电泳法对手性西孟旦对映体的拆分。考察了硼酸盐缓冲溶液的浓度和pH值、背景电解质中β-CD浓度和甲醇含量的影响。将这些因素与以β-CD作为手性流动相添加剂的高效液相色谱法（CMPA-HPLC）中的因素进行了比较。考察了所建立的毛细管电泳方法的定量性能。在含有12 mmol/L β-CD-甲醇（体积比50:50）的20 mmol/L硼酸盐缓冲液（pH 11.0）背景电解质中实现了西孟旦对映体的基线分离。尽管毛细管电泳法的最佳pH值（11.0）远高于CMPA-HPLC中的最佳pH值（6.0），但该毛细管电泳法在手性拆分方面与CMPA-HPLC相当。这种手性毛细管电泳法适用于L-西孟旦的定量分析和对映体过量值的测定。
