基于氯氮平在胶束介质中被溴酸盐氧化的分光光度法测定。

Spectrophotometric determination of clozapine based on its oxidation with bromate in a micellar medium.

作者信息

Mohamed Ashraf A, Al-Ghannam Sheikha M

机构信息

Department of Chemistry, Faculty of Science, King Khalid University, Abha 9033, Saudi Arabia.

出版信息

Farmaco. 2004 Nov;59(11):907-11. doi: 10.1016/j.farmac.2004.07.008.

DOI:10.1016/j.farmac.2004.07.008

PMID:15544796

Abstract

A simple and sensitive spectrophotometric method was developed for the determination of clozapine in its dosage forms. The method is based on the reaction of the drug with potassium bromate in a perchloric acid medium to produce an intense yellow colored species exhibiting a maximum absorption at 308 nm. Beer's law is obeyed for up to 12.0 microg ml(-1) with a correlation coefficient (n = 6) of 0.9998 and a detection limit (3S(b)) of 0.1 microg ml(-1). The molar absorptivity is 1.986 x 10(4) l mol(-1) cm(-1). The various experimental parameters affecting the development and stability of the colored oxidation product were carefully studied and optimized. The proposed method was successfully applied to the determination of clozapine in its dosage forms. The results obtained were in good agreement with those obtained using the B.P. official method.

摘要

建立了一种简单灵敏的分光光度法用于测定氯氮平制剂中的氯氮平。该方法基于药物在高氯酸介质中与溴酸钾反应生成一种在308nm处有最大吸收的强烈黄色物质。在高达12.0μg ml⁻¹范围内符合比尔定律，相关系数（n = 6）为0.9998，检测限（3S(b)）为0.1μg ml⁻¹。摩尔吸光系数为1.986×10⁴ l mol⁻¹ cm⁻¹。仔细研究并优化了影响有色氧化产物生成和稳定性的各种实验参数。所提出的方法成功应用于氯氮平制剂中氯氮平的测定。所得结果与采用英国药典官方方法得到的结果吻合良好。