Heilig Markus, Sommer Wolfgang
Division of Psychiatry, NEUROTEC Department, Karolinska Institutet, M57 Huddinge University Hospital, S-141 86 Stockholm, Sweden.
Neurotox Res. 2004;6(5):363-72. doi: 10.1007/BF03033310.
Genetic factors contribute to alcohol dependence through two main categories of mechanisms. The 50-60% heritability observed in this disorder is presumably conferred by polymorphic variants, encoding functionally altered proteins, or leading to differential transcriptional activity. Secondly, long term changes during the process of developing dependence are likely encoded by persistent changes in gene expression. Thus, genetic and environmental factors interact at the level of the transcriptome, making this an attractive level of analysis. For this purpose, we have applied differential display and more recently Affymetrix oligonucleotide gene arrays to models of genetic susceptibility and alcohol-induced neuroadaptation.
遗传因素通过两类主要机制导致酒精依赖。在这种疾病中观察到的50%-60%的遗传力可能由多态性变体赋予，这些变体编码功能改变的蛋白质，或导致转录活性差异。其次，在依赖形成过程中的长期变化可能由基因表达的持续变化编码。因此，遗传和环境因素在转录组水平上相互作用，使其成为一个有吸引力的分析层面。为此，我们已将差异显示技术以及最近的Affymetrix寡核苷酸基因芯片应用于遗传易感性和酒精诱导的神经适应模型。
