通过絮凝和免疫磁分离法检测游泳池过滤反冲洗水浓缩物中的隐孢子虫卵囊和贾第虫包囊。

Detection of Cryptosporidium oocysts and Giardia cysts in swimming pool filter backwash water concentrates by flocculation and immunomagnetic separation.

作者信息

Greinert Juliane, Furtado Danielle, Smith James, Monte Barardi Célia, Simões Cláudia

机构信息

Departamento de Microbiologia e Parasitologia MIP, CCB, LaboratOrio de Virologia Aplicada Universidade Federal de Santa Catarina Brazil.

出版信息

Int J Environ Health Res. 2004 Dec;14(6):395-404. doi: 10.1080/09603120400012892.

DOI:10.1080/09603120400012892

PMID:15545035

Abstract

The purpose of the present study was to evaluate techniques for detection of Giardia cysts and Cryptosporidium oocysts in swimming pool filter backwash water. Calcium carbonate flocculation was used for water samples concentration of 1 l filter backwash water samples. Immunomagnetic separation (IMS) was used for separation of cysts and oocysts from detrital material. Cysts and oocysts were then detected using direct immunofluorescence. ColorSeed C&G was used as an internal standard. Recoveries were examined at several processing points. Highest recoveries (23% Cryptosporidium, 18% Giardia) were obtained using lower filter backwash volumes, greater IMS volumes, and addition of Tween20 to backwash samples prior to processing. The combination of CaCO3 flocculation, IMS and direct immunofluorescence was found to be an effective tool for the detection and quantification of Giardia spp. and Cryptosporidium spp. in filter backwash water samples.

摘要

本研究的目的是评估检测游泳池过滤反冲洗水中贾第虫包囊和隐孢子虫卵囊的技术。采用碳酸钙絮凝法对1升过滤反冲洗水样进行水样浓缩。免疫磁珠分离（IMS）用于从碎屑物质中分离包囊和卵囊。然后使用直接免疫荧光法检测包囊和卵囊。ColorSeed C&G用作内标。在几个处理点检测回收率。使用较低的过滤反冲洗体积、较大的IMS体积以及在处理前向反冲洗样品中添加吐温20可获得最高回收率（隐孢子虫为23%，贾第虫为18%）。发现碳酸钙絮凝、IMS和直接免疫荧光的组合是检测和定量过滤反冲洗水样中贾第虫属和隐孢子虫属的有效工具。