Houman Habib, Hamzaoui Agnes, Ben Ghorbal Imed, Khanfir Monia Smiti, Feki Moncef, Hamzaoui Kamel
Department of Internal Medicine, La Rabta Hospital, Tunis, Tunisia.
Mediators Inflamm. 2004 Aug;13(4):247-53. doi: 10.1080/09629350400003167.
Peripheral blood CD8+ T cells expressing interferon gamma and interleukin-4 (IL-4), and lacking CD28 molecules, were responsible for the dynamic interplay between peripheral blood and inflammatory sites.
The aim of the current study was to define in Behçet's disease (BD), CD8+ T-cell subsets using CD28 and CD11b monoclonal antibodies, and the characterization of the Tc1/Tc2 ratio and perforin expression.
Flow cytometry was used for intracytoplasmic cytokines and perforin expression. Effector cells were investigated by adhesion of CD8+ T cells to human microvascular endothelial cells and by chemotaxis using beta-chemokine.
Interferon-gamma-producing CD8+ T cells in active and remission BD patients were increased, which induce a significant increase of the Tc1:Tc2 ratio in BD. CD8(+)CD28(-)CD11b+ T cells were found to be more expanded in BD patients than in age-matched healthy controls. The expression of CD11b molecules in active BD allowed to CD8(+)CD28+/CD8(+)CD28- subsets to adhere to human microvascular endothelial cells, with more efficiency in BD. Using MIP-1alpha, we observed that the migratory process of CD28(-)CD11b(+) is more important in BD. CD28(-)CD11b+ exhibited an increased perforin expression in BD patients.
Taken together these results suggest the presence of immune activation, probably in response to a profound inflammation affecting BD patients. The physiopathological significance of these results were toward autoimmune diseases and/or infectious process.
表达干扰素γ和白细胞介素-4(IL-4)且缺乏CD28分子的外周血CD8+ T细胞,参与了外周血与炎症部位之间的动态相互作用。
本研究的目的是利用CD28和CD11b单克隆抗体确定白塞病(BD)中CD8+ T细胞亚群,并对Tc1/Tc2比值和穿孔素表达进行特征分析。
采用流式细胞术检测细胞内细胞因子和穿孔素表达。通过CD8+ T细胞与人微血管内皮细胞的黏附以及使用β趋化因子的趋化作用来研究效应细胞。
活动期和缓解期BD患者中产生干扰素γ的CD8+ T细胞增加,这导致BD中Tc1:Tc2比值显著升高。发现BD患者中CD8(+)CD28(-)CD11b+ T细胞比年龄匹配的健康对照者扩增得更多。活动期BD中CD11b分子的表达使CD8(+)CD28+/CD8(+)CD28-亚群能够与人微血管内皮细胞黏附,在BD中效率更高。使用MIP-1α,我们观察到CD28(-)CD11b(+)的迁移过程在BD中更为重要。BD患者中CD28(-)CD11b+的穿孔素表达增加。
综合这些结果表明存在免疫激活,可能是对影响BD患者的严重炎症的反应。这些结果的生理病理学意义涉及自身免疫性疾病和/或感染过程。