Houman Habib, Hamzaoui Agnès, Ben Ghorbal Imed, Khanfir Monia, Feki Moncef, Hamzaoui Kamel
Department of Internal Medicine, La Rabta Hospital, Tunis, Tunisia.
J Autoimmun. 2004 Nov;23(3):267-73. doi: 10.1016/j.jaut.2004.07.005.
Dynamic interplay between cytokines and chemokines directs trafficking of peripheral blood mononuclear cells to tissues in autoimmune and/or viral diseases. The aim of the current study was to define the expression on CD3+ T cells of six chemokine receptors associated with inflammatory sites and the expression of intracellular cytokines, such as interferon-gamma (IFN-gamma) and interleukin-4 (IL-4), in Behcet's disease (BD). Flow cytometry was used to detect chemokine receptor and intracytoplasmic cytokines' expression. We observed that CD3+ T cells in the peripheral blood express a restricted array of inflammatory chemokine receptors, specifically, CCR5, CCR6 and CXCR3, but little CCR1-3. The highest expression of CXCR3 on CD3+ T cells is associated with the presence of central nervous system (CNS) manifestations or pulmonary involvement. CXCR3 is the principal inflammatory chemokine receptor involved in BD. CCR5 chemokine receptor is increased in BD regardless of clinical manifestations. The frequency of IFN-gamma-producing cells expressing CXCR3+ CD3+ cells is significantly increased in patients with BD compared with normal controls. IL-4-producing cells are decreased in BD. These results demonstrate the predominance of type 1 cytokine producing cells in CXCR3+ CD3+ T cells during BD. We hypothesize that CXCR3 is the principal inflammatory chemokine receptor involved in BD, particularly during CNS and pulmonary manifestations.
细胞因子和趋化因子之间的动态相互作用引导外周血单核细胞在自身免疫性疾病和/或病毒性疾病中向组织迁移。本研究的目的是确定与炎症部位相关的六种趋化因子受体在白塞病（BD）患者CD3⁺T细胞上的表达情况，以及细胞内细胞因子如干扰素-γ（IFN-γ）和白细胞介素-4（IL-4）的表达情况。采用流式细胞术检测趋化因子受体和细胞内细胞因子的表达。我们观察到，外周血中的CD3⁺T细胞表达一系列受限的炎症趋化因子受体，具体而言，CCR5、CCR6和CXCR3，但CCR1-3表达较少。CD3⁺T细胞上CXCR3的最高表达与中枢神经系统（CNS）表现或肺部受累有关。CXCR3是参与白塞病的主要炎症趋化因子受体。无论临床表现如何，白塞病患者CCR5趋化因子受体均增加。与正常对照相比，白塞病患者中表达CXCR3⁺CD3⁺细胞的IFN-γ产生细胞频率显著增加。白塞病患者中产生IL-4的细胞减少。这些结果表明，在白塞病期间，CXCR3⁺CD3⁺T细胞中以产生1型细胞因子的细胞为主。我们推测，CXCR3是参与白塞病的主要炎症趋化因子受体，尤其是在中枢神经系统和肺部表现期间。
