Suh Sung-Oui, McHugh Joseph V, Blackwell Meredith
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Department of Entomology, University of Georgia, Athens, GA 30602, USA.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2409-2429. doi: 10.1099/ijs.0.63246-0.
A major clade of new yeast taxa from the digestive tract of basidiocarp-feeding beetles is recognized based on rRNA gene sequence analyses. Almost 30 % of 650 gut isolates formed a statistically well-supported clade that included Candida tanzawaensis. The yeasts in the clade were isolated from 11 families of beetles, of which Tenebrionidae and Erotylidae were most commonly sampled. Repeated isolation of certain yeasts from the same beetle species at different times and places indicated strong host associations. Sexual reproduction was never observed in the yeasts. Based on comparisons of small- and large-subunit rRNA gene sequences and morphological and physiological traits, the yeasts were placed in Candida ambrosiae and in 16 other undescribed taxa. In this report, the novel species in the genus Candida are described and their relationships with other taxa in the Saccharomycetes are discussed. The novel species and their type strains are as follows: Candida guaymorum (NRRL Y-27568(T)=CBS 9823(T)), Candida bokatorum (NRRL Y-27571(T)=CBS 9824(T)), Candida kunorum (NRRL Y-27580(T)=CBS 9825(T)), Candida terraborum (NRRL Y-27573(T)=CBS 9826(T)), Candida emberorum (NRRL Y-27606(T)=CBS 9827(T)), Candida wounanorum (NRRL Y-27574(T)=CBS 9828(T)), Candida yuchorum (NRRL Y-27569(T)=CBS 9829(T)), Candida chickasaworum (NRRL Y-27566(T)=CBS 9830(T)), Candida choctaworum (NRRL Y-27584(T)=CBS 9831(T)), Candida bolitotheri (NRRL Y-27587(T)=CBS 9832(T)), Candida atakaporum (NRRL Y-27570(T)=CBS 9833(T)), Candida panamericana (NRRL Y-27567(T)=CBS 9834(T)), Candida bribrorum (NRRL Y-27572(T)=CBS 9835(T)), Candida maxii (NRRL Y-27588(T)=CBS 9836(T)), Candida anneliseae (NRRL Y-27563(T)=CBS 9837(T)) and Candida taliae (NRRL Y-27589(T)=CBS 9838(T)).
基于rRNA基因序列分析，确认了一个来自取食担子果甲虫消化道的新酵母类群的主要分支。在650株肠道分离菌株中，近30%形成了一个在统计学上得到有力支持的分支，其中包括淡路假丝酵母（Candida tanzawaensis）。该分支中的酵母从11个甲虫科中分离得到，其中拟步甲科（Tenebrionidae）和红瓢虫科（Erotylidae）是采样最频繁的科。在不同时间和地点从同一甲虫物种中反复分离出某些酵母，表明它们与宿主有很强的关联。从未在这些酵母中观察到有性繁殖。基于小亚基和大亚基rRNA基因序列以及形态和生理特征的比较，这些酵母被归入安布罗西亚假丝酵母（Candida ambrosiae）和其他未描述的16个分类单元。在本报告中，描述了假丝酵母属中的新物种，并讨论了它们与酵母菌纲中其他分类单元的关系。新物种及其模式菌株如下：瓜伊莫尔假丝酵母（Candida guaymorum，NRRL Y - 27568(T)=CBS 9823(T)）、博卡托假丝酵母（Candida bokatorum，NRRL Y - 27571(T)=CBS 9824(T)）、库诺假丝酵母（Candida kunorum，NRRL Y - 27580(T)=CBS 9825(T)）、特拉博假丝酵母（Candida terraborum，NRRL Y - 27573(T)=CBS 9826(T)）、恩贝假丝酵母（Candida emberorum，NRRL Y - 27606(T)=CBS 9827(T)）、沃南假丝酵母（Candida wounanorum，NRRL Y - 27574(T)=CBS 9828(T)）、玉楚假丝酵母（Candida yuchorum，NRRL Y - 27569(T)=CBS 9829(T)）、奇卡索假丝酵母（Candida chickasaworum，NRRL Y - 27566(T)=CBS 9830(T)）、乔克托假丝酵母（Candida choctaworum，NRRL Y - 27584(T)=CBS 9831(T)）、博利托假丝酵母（Candida bolitotheri，NRRL Y - 27587(T)=CBS 9832(T)）、阿塔卡波假丝酵母（Candida atakaporum，NRRL Y - 27570(T)=CBS 9833(T)）、泛美假丝酵母（Candida panamericana，NRRL Y - 27567(T)=CBS 9834(T)）、布里布罗假丝酵母（Candida bribrorum，NRRL Y - 27572(T)=CBS 9835(T)）、马克西假丝酵母（Candida maxii，NRRL Y - 27588(T)=CBS 9836(T)）、安妮莉丝假丝酵母（Candida anneliseae，NRRL Y - 27563(T)=CBS 9837(T)）和塔利亚假丝酵母（Candida taliae，NRRL Y - 27589(T)=CBS 9838(T)）。
