Knight J A, Stephens R W, Bushell G R, Ghosh P, Taylor T K
Biochim Biophys Acta. 1979 May 1;584(2):304-10. doi: 10.1016/0304-4165(79)90276-9.
Assays of several proteases, incorporating guanidinium chloride extracts of human femoral head cartilage and intervertebral disc, demonstrated that both tissues contain inhibitors of certain serine proteases. Trypsin, chymotrypsin and a granule extract of human polymorphonuclear leukocytes containing elastase and cathepsin G activities, were inhibited by low molecular weight fractions prepared by Sephadex G-75 chromatography. Using a radioassay, it was further shown that these fractions inhibit proteolysis of cartilage proteoglycan. The inhibitor in intervertebral disc is concentrated in the nucleus pulposus, with a decreasing gradient to the periphery of the annulus fibrosus. It is proposed that these inhibitors confer at least partial protection against pathological proteolysis of the proteoglycans in human articular cartilage and nucleus pulposus.
对几种蛋白酶进行的检测，采用人股骨头软骨和椎间盘的氯化胍提取物，结果表明这两种组织都含有某些丝氨酸蛋白酶的抑制剂。胰蛋白酶、胰凝乳蛋白酶以及含有弹性蛋白酶和组织蛋白酶G活性的人多形核白细胞颗粒提取物，都被通过Sephadex G - 75色谱法制备的低分子量组分所抑制。通过放射性检测进一步表明，这些组分抑制软骨蛋白聚糖的蛋白水解。椎间盘内的抑制剂集中在髓核中，向纤维环周边呈递减梯度。有人提出，这些抑制剂至少能部分保护人关节软骨和髓核中的蛋白聚糖免受病理性蛋白水解。
