人类椎间盘的蛋白聚糖
The proteoglycans of the human intervertebral disc.
作者信息
Emes J H, Pearce R H
出版信息
Biochem J. 1975 Mar;145(3):549-56. doi: 10.1042/bj1450549.
Abstract
The methods of Hascall & Sajdera (1969) were used to compare the proteoglycans of human intervertebral disc with those of bovine nasal cartilage. In contrast with cartilage, most of the hexuronate of disc could be extracted at low shear with water or dilute salt solutions. Extracts of disc with 4M-guanidinium chloride were centrifugated in 0.4M-guanidinium chloride in a CsCl gradient. Analytical ultracentrifugation of the hexuronate-containing heavy component revealed two fractions. both more polydisperse than those of cartilage. Also the more rapidly sediminting component was a much smaller fraction of the total. After prior extraction with 0.4M-guanidinium chloride, 4M-guanidinium chloride extracts of disc were found, by ultracentrifugal analysis, to be enriched in components resembling the proteoglycan monomer and aggregating factors of cartilage.
摘要
采用哈斯卡尔和萨伊德拉（1969年）的方法，将人类椎间盘的蛋白聚糖与牛鼻软骨的蛋白聚糖进行比较。与软骨不同的是，椎间盘的大部分己糖醛酸可以在低剪切力下用水或稀盐溶液提取。用4M氯化胍提取的椎间盘提取物在含有0.4M氯化胍的氯化铯梯度中进行离心。对含己糖醛酸的重组分进行分析超速离心，结果显示有两个组分，二者的多分散性均高于软骨中的组分。此外，沉降较快的组分在总量中所占比例要小得多。在用0.4M氯化胍预先提取后，通过超速离心分析发现，椎间盘的4M氯化胍提取物富含类似于软骨蛋白聚糖单体和聚集因子的组分。
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