Nagao Tamiko, Yoshida Akiko, Sakurai Akiko, Piroozmand Ahmad, Jere Abhay, Fujita Mikako, Uchiyama Tsuneo, Adachi Akio
Department of Virology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan.
Int J Mol Med. 2004 Dec;14(6):1073-6.
We have established lymphocytic cell lines H9 and M8166 that contain integrated copy of luciferase gene under the control of human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR). While H9 is known to be non-permissive for or insensitive to some particular mutant strains of HIV/simian immunodeficiency virus (SIV), M8166 is one of the most susceptible lines to various HIV/SIVs. The luciferase gene driven by HIV-1 LTR was transfected into H9 and M8166 cells with the neo gene, and cell lines were selected by G418. The indicator cell lines thus obtained were designated H9/H1luc and M8166/H1luc, and monitored for their susceptibility to various HIV clones including in vitro-constructed mutants. Both cell lines, particularly M8166/H1luc, were found to be exquisitely sensitive to HIV-1 and HIV-2. Furthermore, they exhibited the response to infections by various viral clones exactly as expected from the characteristics of the original cell lines. These results indicated that our new indicator cell lines H9/H1luc and M8166/H1luc are eminently useful for a variety of molecular virological studies on HIV/SIV.
我们建立了淋巴细胞系H9和M8166，它们含有在人类免疫缺陷病毒1型（HIV-1）长末端重复序列（LTR）控制下的整合型荧光素酶基因拷贝。虽然已知H9对某些特定的HIV/猴免疫缺陷病毒（SIV）突变株不敏感或无反应，但M8166是对各种HIV/SIV最敏感的细胞系之一。将由HIV-1 LTR驱动的荧光素酶基因与新霉素基因一起转染到H9和M8166细胞中，并用G418筛选细胞系。由此获得的指示细胞系命名为H9/H1luc和M8166/H1luc，并监测它们对包括体外构建的突变体在内的各种HIV克隆的敏感性。发现这两种细胞系，尤其是M8166/H1luc，对HIV-1和HIV-2极其敏感。此外，它们对各种病毒克隆感染的反应与原始细胞系的特征完全一致。这些结果表明，我们新的指示细胞系H9/H1luc和M8166/H1luc对于HIV/SIV的各种分子病毒学研究非常有用。
