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Conversion from arenes having a benzene ring to those having a picolinic acid by simple growing cell reactions using Escherichia coli that expressed the six bacterial genes involved in biphenyl catabolism.

作者信息

Shindo Kazutoshi, Osawa Ayako, Nakamura Ryoko, Kagiyama Yukiko, Sakuda Shohei, Shizuri Yoshikazu, Furukawa Kensuke, Misawa Norihiko

机构信息

Department of Food and Nutrition, Japan Women's University, 2-8-1, Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan.

出版信息

J Am Chem Soc. 2004 Nov 24;126(46):15042-3. doi: 10.1021/ja044850g.

DOI:10.1021/ja044850g
PMID:15547997
Abstract

The comprehensive bioconversion of aromatic compounds with a benzene ring to a picolinic acid was achieved with a recombinant Escherichia coli strain that expressed the six genes involved in biphenyl catabolism, these being the bphA1(2072)A2A3A4 genes encoding the evolved biphenyl dioxygenase, the bphB gene encoding dihydrodiol dehydrogenase, and the bphC gene encoding catechol 2,3-dioxygenase.

摘要

相似文献

1
Conversion from arenes having a benzene ring to those having a picolinic acid by simple growing cell reactions using Escherichia coli that expressed the six bacterial genes involved in biphenyl catabolism.
J Am Chem Soc. 2004 Nov 24;126(46):15042-3. doi: 10.1021/ja044850g.
2
Substitution of the ISP alpha subunit of biphenyl dioxygenase from Pseudomonas results in a modification of the enzyme activity.假单胞菌联苯双加氧酶的ISPα亚基的替换导致酶活性的改变。
Biochem Biophys Res Commun. 1994 Oct 28;204(2):912-7. doi: 10.1006/bbrc.1994.2546.
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Heterologous expression of biphenyl dioxygenase-encoding genes from a gram-positive broad-spectrum polychlorinated biphenyl degrader and characterization of chlorobiphenyl oxidation by the gene products.来自革兰氏阳性广谱多氯联苯降解菌的联苯双加氧酶编码基因的异源表达及基因产物对氯联苯氧化的特性研究
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