Schultheiss D, Gabouev A I, Kaufmann P M, Schlote N, Mertsching H, Haverich A, Stief C G, Jonas U
Klinik und Poliklinik für Urologie und Kinderurologie, Medizinische Hochschule Hannover.
Urologe A. 2004 Oct;43(10):1223-8. doi: 10.1007/s00120-004-0702-7.
A new technique is presented to harvest an acellular matrix from a porcine small bowel segment preserving the mesenteric arterial and venous pedicles. Reseeding of this biological vascularized matrix (BioVaM) with functional cells, i.e. smooth muscle and urothelial cells isolated from the urinary tract, and resurfacing of its vascular structures with endothelial precursor cells results in a vascularized tissue engineered graft for reconstruction and augmentation of the urinary bladder. First promising short term implantation experiments using a porcine model for the evaluation of early graft perfusion after vascular anastomosis are presented.
提出了一种新技术，用于从保留肠系膜动静脉蒂的猪小肠段获取无细胞基质。用功能细胞，即从尿路分离的平滑肌和尿路上皮细胞对这种生物血管化基质（BioVaM）进行再接种，并用内皮祖细胞对其血管结构进行表面修复，从而形成用于膀胱重建和扩大的血管化组织工程移植物。介绍了使用猪模型进行的首次有前景的短期植入实验，以评估血管吻合术后早期移植物灌注情况。
