Wilhjelm J E, Jensen M S, Gammelmark K L, Sahl B, Martinsen K, Hansen J U, Brandt T, Jespersen S K, Falk E, Fredfeldt K E, Sillesen H
Center for Arteriosclerosis Detection with Ultrasound (CADUS), Ørsted.DTU, Technical University of Denmark, Kgs. Lyngby, Denmark.
Ultrasound Med Biol. 2004 Sep;30(9):1119-31. doi: 10.1016/j.ultrasmedbio.2004.07.011.
Ten formalin-fixed atherosclerotic carotid plaques removed by endarterectomy were molded into rectangular agar blocks containing fiducial markers on the top surface. Plaque and fiducial markers were imaged with 3-D multiangle ultrasound (US) spatial compounding as well as planar X ray. Subsequently, the blocks were decalcified, sliced, photographed and analyzed histologically. This gave a total of 123 slices. The plaque regions of the photographs were outlined and the outline adjusted to partly compensate for occasional displacement during slicing. Inside this outline, the material constitutions were found by incorporating the histologic information. From this set, slices with 1. too much tissue displacement due to cutting or 2. lack of identification of calcification as found by x ray, were removed. This resulted in 53 reference maps. The material types identified covered soft tissues, fibrous tissue, calcified tissue and unidentified tissues. The 53 reference maps can be used for direct automated quantitative comparison with US images.
通过动脉内膜切除术移除的10个经福尔马林固定的动脉粥样硬化颈动脉斑块被制成矩形琼脂块，其顶面含有基准标记。使用三维多角度超声（US）空间复合成像以及平面X射线对斑块和基准标记进行成像。随后，将这些块脱钙、切片、拍照并进行组织学分析。总共得到了123个切片。对照片中的斑块区域进行勾勒，并对轮廓进行调整，以部分补偿切片过程中偶尔出现的位移。在这个轮廓内，通过纳入组织学信息来确定材料组成。从这个集合中，移除了1. 由于切割导致组织位移过多或2. X射线未发现钙化的切片。这产生了53个参考图谱。所识别的材料类型包括软组织、纤维组织、钙化组织和未识别组织。这53个参考图谱可用于与超声图像进行直接自动定量比较。
