Alcántara S, Velasco A, Revah S
Instituto Mexicano del Petróleo, Programa de Biotecnología del Petróleo, Eje Central Lázaro Cárdenas 152, C.P. 07730 México DF.
Environ Technol. 2004 Oct;25(10):1151-7. doi: 10.1080/09593330.2004.9619409.
The elemental sulfur formation by the partial oxidation of thiosulfate by both a sulfoxidizing consortium and by Thiobacillus thioparus ATCC 23645 was studied under aerobic conditions in chemostat. Steady state was attained with essentially total conversion to sulfate when the dissolved oxygen concentration was 5 mgO2 l(-1) and below a dilution rate (D) of 3.0 d(-1)for the consortium and 0.9 d(-1) for T thioparus. The consortium formed elemental sulfur in steady state under oxygen limitation. Fifty percent of the theoretical elemental sulfur yield was obtained with a dissolved oxygen concentration of 0.2 mgO2 l(-1). Growth of T thioparus was negatively affected with a concentration below 1.9 mgO2 l(-1). Consortium yield from batch cultures was 2.1 g(-1) (protein) mol(-1) (thiosulfate), which was comparable with the values obtained in the chemostat at dilution rates of 0.4 d(-1) and 1.2 d(-1). The consortium showed a maximum degradation rate of 0.105 g(thiosulfate) g(-1) (protein) min(-1) and a saturation rate for S2O3(2-) of 1.9 mM.
在恒化器的好氧条件下，研究了硫氧化菌联合体和嗜硫代硫酸盐硫杆菌ATCC 23645通过硫代硫酸盐部分氧化形成元素硫的过程。当溶解氧浓度为5 mgO₂ l⁻¹且联合体的稀释率（D）低于3.0 d⁻¹、嗜硫代硫酸盐硫杆菌的稀释率低于0.9 d⁻¹时，可实现基本完全转化为硫酸盐的稳态。在氧气限制条件下，联合体在稳态时形成元素硫。溶解氧浓度为0.2 mgO₂ l⁻¹时，可获得理论元素硫产量的50%。溶解氧浓度低于1.9 mgO₂ l⁻¹时，嗜硫代硫酸盐硫杆菌的生长受到负面影响。分批培养中联合体的产量为2.1 g⁻¹（蛋白质）mol⁻¹（硫代硫酸盐），这与在稀释率为0.4 d⁻¹和1.2 d⁻¹的恒化器中获得的值相当。联合体的最大降解速率为0.105 g（硫代硫酸盐）g⁻¹（蛋白质）min⁻¹，S₂O₃²⁻的饱和速率为1.9 mM。
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