大肠杆菌素浓度在体外抑制大肠杆菌O157:H7的生长。

Colicin concentrations inhibit growth of Escherichia coli O157:H7 in vitro.

作者信息

Callaway T R, Stahl C H, Edrington T S, Genovese K J, Lincoln L M, Anderson R C, Lonergan S M, Poole T L, Harvey R B, Nisbet D J

机构信息

Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, College Station, Texas 77845, USA.

出版信息

J Food Prot. 2004 Nov;67(11):2603-7. doi: 10.4315/0362-028x-67.11.2603.

DOI:10.4315/0362-028x-67.11.2603

PMID:15553648

Abstract

Escherichia coli O157:H7 is a virulent foodborne pathogen that causes severe human illness and inhabits the intestinal tract of food animals. Colicins are antimicrobial proteins produced by E. coli strains that inhibit or kill other E. coli. In the present Study, the efficacy of three pore-forming colicins (El, N, and A) were quantified in vitro against E. coli O157:H7 strains 86-24 and 933. Colicins E1 and N reduced the growth of E. coli O157:H7 strains, but the efficacy of each colicin varied among strains. Colicin E1 was more effective against both strains of E. coli O157:H7 than colicins A and N and reduced (P < 0.05) populations of E. coli O157:H7 at concentrations <0.1 microg/ml. These potent antimicrobial proteins may potentially provide an effective and environmentally sound preharvest strategy to reduce E. coli O157:H7 in food animals.

摘要

大肠杆菌O157:H7是一种致病性食源性病原体，可导致人类严重疾病，并存在于食用动物的肠道中。大肠杆菌素是由大肠杆菌菌株产生的抗菌蛋白，可抑制或杀死其他大肠杆菌。在本研究中，对三种形成孔道的大肠杆菌素（E1、N和A）针对大肠杆菌O157:H7菌株86 - 24和933的体外效力进行了量化。大肠杆菌素E1和N可降低大肠杆菌O157:H7菌株的生长，但每种大肠杆菌素的效力在不同菌株间有所差异。大肠杆菌素E1对两种大肠杆菌O157:H7菌株的效果均优于大肠杆菌素A和N，且在浓度<0.1微克/毫升时可降低（P < 0.05）大肠杆菌O157:H7的数量。这些强效抗菌蛋白可能为减少食用动物体内的大肠杆菌O157:H7提供一种有效且环保的收获前策略。