Dobrov E N, Efimov A V, Baratova L A
Mol Biol (Mosk). 2004 Sep-Oct;38(5):945-58.
The results of the studies of helical plant virus structures by tritium planigraphy (TP) method are discussed. TP method is based on bombardment of macromolecular objects with a stream of tritium atoms, followed by analysis of tritium label distribution along the macromolecule. By combining the TP data with the results of theoretical predictions of the protein structure, it turned out to be possible to propose a model of the coat protein structure in the virions of potato virus X (the type member of potexvirus group) and potato virus A (one of the members of potyvirus group). With the help of TP it also managed to find subtle differences in the coat protein structure between wildtype tobacco mosaic virus (strain U1) and its mutant with two amino acid substitutions in the coat protein and alter host specificity.
讨论了用氚平面成像（TP）方法对螺旋植物病毒结构的研究结果。TP方法基于用氚原子流轰击大分子物体，然后分析氚标记沿大分子的分布情况。通过将TP数据与蛋白质结构的理论预测结果相结合，结果表明有可能提出马铃薯X病毒（马铃薯X病毒属的模式成员）和马铃薯A病毒（马铃薯Y病毒属的成员之一）病毒粒子中衣壳蛋白结构的模型。借助TP还成功地发现了野生型烟草花叶病毒（U1株）与其衣壳蛋白中有两个氨基酸替换且改变宿主特异性的突变体之间衣壳蛋白结构的细微差异。
