Mondal Mohan, Prakash B S
National Research Centre on Mithun (ICAR), Jharnapani, Medziphema, Nagaland, India.
Endocr Res. 2004 Aug;30(3):301-13. doi: 10.1081/erc-200033182.
To assess the changes of plasma growth hormone (GH) and secretion patterns of GH and luteinizing hormone (LH) during growth in buffaloes, six growing female Murrah buffalo calves (mean age 6+/-0.9 months and body weight 66+/-6 kg) were selected. Plasma samples were collected twice a week for 52 weeks for GH and LH assay. To examine for pulsatile secretion samples were collected at 15 minutes interval for 9 hr at weeks 6 and 42 for GH and LH measurements. Plasma progesterone was also estimated in twice-a-week samples to assess whether any of the buffalo had begun ovarian cyclicity. The body weight of all animals was recorded at weekly interval. Plasma GH concentration decreased (P < 0.01) only up to week 29 and showed an increasing trend (P < 0.01) thereafter. The ratio of plasma GH to body weight declined (P < 0.01) throughout the entire experimental period. Plasma GH showed a declining trend only up to when the animals attained 155 kg body weight and thereafter showed an increasing trend (P < 0.01). Plasma GH revealed distinct pulsatile patterns of release, with a mean of 6 and 5 pulses in the 6-week and 42-week samples, respectively. The plasma LH concentrations around the 42-week time period were significantly higher (P < 0.01) than at the 6-week time period, and they exhibited pulsatility. No animal reached puberty until the end of the experiment. In summary, plasma GH levels have a definite pattern of change during growth and patterns of secretion of plasma GH and LH also have a relation with body weight in this species of animal.
为评估水牛生长过程中血浆生长激素（GH）的变化以及GH和促黄体生成素（LH）的分泌模式，选取了6头生长中的雌性摩拉水牛犊牛（平均年龄6±0.9个月，体重66±6千克）。每周采集两次血浆样本，持续52周，用于检测GH和LH。为检测脉冲式分泌，在第6周和第42周每隔15分钟采集一次样本，持续9小时，用于测量GH和LH。每周两次的样本还用于检测血浆孕酮，以评估是否有任何水牛开始出现卵巢周期性变化。每周记录所有动物的体重。血浆GH浓度仅在第29周之前下降（P<0.01），此后呈上升趋势（P<0.01）。在整个实验期间，血浆GH与体重的比值下降（P<0.01）。血浆GH仅在动物体重达到155千克之前呈下降趋势，此后呈上升趋势（P<0.01）。血浆GH呈现出明显的脉冲式释放模式，在第6周和第42周的样本中，平均脉冲数分别为6次和5次。第42周左右的血浆LH浓度显著高于第6周（P<0.01），且呈现出脉冲性。直到实验结束，没有动物达到青春期。总之，在这种动物中，血浆GH水平在生长过程中有明确的变化模式，血浆GH和LH的分泌模式也与体重有关。
