[γ-干扰素对实验性矽肺大鼠白细胞介素-4及转化生长因子-β1蛋白表达的影响]

[Effect of gamma-interferon on protein expressions of interleukin-4 and transforming growth factor-beta1 in experimental silicotic rats].

作者信息

Chen Ying, Dong Jing, Chen Jie

机构信息

Division of Pneumoconiosis, School of Public Health, China Medical University, Shenyang 110001, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2004 Oct;22(5):350-3.

PMID:15555310

Abstract

OBJECTIVE

To study the effect of gamma-interferon on the protein expressions of interleukin-4 (IL-4) and transforming growth factor-beta(1)(TGF-beta1) on silicotic rats.

METHODS

Wistar rats were exposed to lower dose (20 mg) of quartz by intratracheal injection. Next day, the experimental rats were treated with gamma-interferon (1 x 10(5) IU) daily. Rats were killed in 1 month and 2 month to observe the changes of pathology of lung tissue by use of HE and VG staining, and the expressions of IL-4 and TGF-beta1 by use of immunohistochemical technique and quantitative analysis of protein expressions by image analysis system.

RESULTS

(1) The fibrotic degree in silicotic lesions of rat lung was 1/2 approximately 1 grade less in IFN-gamma treated groups than in quartz controls. (2) Quantitative image analysis showed that the integrated optical density (IOD) of IL-4 positive cell in quartz controls in 1 month was (3.33 +/- 1.27) x 10(4) and decreased to (1.99 +/- 0.80) x 10(4) in 2 month, and that of TGF-beta1 positive cell increased from (3.67 +/- 0.63) x 10(4) to (4.90 +/- 1.11) x 10(4); The IOD of IL-4 positive cell in IFN-gamma treated groups was decreased from (2.19 +/- 0.90) x 10(4) to (0.61 +/- 0.22) x 10(4)(P < 0.05), and that of TGF-beta1 positive cell increased from (1.37 +/- 0.31) x 10(4) to (1.76 +/- 0.72) x 10(4) (P < 0.05). At these two time points, the IODof IL-4 and TGF-beta1 positive cell in IFN-gamma treated groups were lower than those in quartz controls (P < 0.05 or P < 0.01).

CONCLUSION

gamma-interferon may inhibit the expressions of IL-4 and TGF-beta1 in silicotic rats.

摘要

目的

研究γ-干扰素对矽肺大鼠白细胞介素-4（IL-4）和转化生长因子-β1（TGF-β1）蛋白表达的影响。

方法

将Wistar大鼠经气管内注射低剂量（20mg）石英。次日，对实验大鼠每日给予γ-干扰素（1×10⁵IU）。分别于1个月和2个月处死大鼠，采用HE和VG染色观察肺组织病理变化，采用免疫组化技术观察IL-4和TGF-β1的表达，并通过图像分析系统对蛋白表达进行定量分析。

结果

（1）γ-干扰素治疗组大鼠肺矽肺病变的纤维化程度比石英对照组减轻约1/2至1级。（2）图像定量分析显示，石英对照组1个月时IL-4阳性细胞的积分光密度（IOD）为（3.33±1.27）×10⁴，2个月时降至（1.99±0.80）×10⁴，TGF-β1阳性细胞的IOD从（3.67±0.63）×10⁴增加至（4.90±1.11）×10⁴；γ-干扰素治疗组IL-4阳性细胞的IOD从（2.19±0.90）×10⁴降至（0.61±0.22）×10⁴（P<0.05），TGF-β1阳性细胞的IOD从（1.37±0.31）×10⁴增加至（1.76±0.72）×10⁴（P<0.05）。在这两个时间点，γ-干扰素治疗组IL-4和TGF-β1阳性细胞的IOD均低于石英对照组（P<0.05或P<0.01）。