早期生长反应基因-1在大鼠实验性矽肺中的表达及作用

[Expression and role of early growth response gene-1 in experimental silicosis of rat].

作者信息

Chu Ling, Li Xiang, Hu Yong-bin, Wang Jin-sheng, Zheng Hui, Zeng Qing-fu

机构信息

Department of Pathology, Xiangya School of Medicine, Central South University, Changsha 410078, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2004 Feb;22(1):47-50.

PMID:15033019

Abstract

OBJECTIVE

To study the expression and location of early growth response gene-1 (Egr-1), transforming growth factor-beta(1) (TGF-beta(1)), fibronectin (FN) in silicotic rat and to discuss the role of Egr-1 in the development of silicosis.

METHODS

Silicotic animal model of rat was established, and the expressions of Egr-1, TGF-beta(1), FN in various lung cells of silicotic rat were analysed by using immunohistochemical technique (SP) and the image analysis.

RESULTS

The expressions of Egr-1 in bronchial epithelial cell, pulmonary macrophage, alveolar epithelium cell and interstitial cell in lung silicotic tissue (gray values: 118.58 +/- 5.65 - 168.52 +/- 5.67) were higher than those of controls (gray values: 166.23 +/- 5.23 - 188.12 +/- 8.35) during 1 - 28 days, and the expression was mainly in nucleus; the expressions of TGF-beta(1) in these cells (gray values: 123.49 +/- 5.65 - 170.24 +/- 3.56) were also higher than those of controls (166.53 +/- 6.25 - 198.56 +/- 4.53), and the expression was mainly in cytoplasm. The expressions of FN in bronchial epithelial cell, pulmonary macrophage and alveolar epithelial cell (gray values: 150.32 +/- 6.54 - 201.54 +/- 7.38) were lower, while those in interstitial cell (gray values: 121.43 +/- 5.65 - 167.55 +/- 6.35) were higher than those of controls. The changes of TGF-beta(1) and Egr-1 expression level in bronchial epithelial cell, pulmonary macrophage, alveolar epithelium cell and interstitial cell were synchronous during the experiment (1 - 28 days). Both of them were correlated with each other (r = 0.61, P < 0.01), while the expression of FN was not correlated with Egr-1, but correlated to TGF-beta(1) in interstitial cell (r = 0.46, P < 0.01).

CONCLUSION

Silicon dioxide could up-regulate the expression of nuclear transcription factor Egr-1 in several kinds of cell in lung. The activated Egr-1 may coordinate the expression of TGF-beta(1) and FN to regulate the development of silicosis.

摘要

目的

研究早期生长反应基因-1（Egr-1）、转化生长因子-β(1)（TGF-β(1)）、纤连蛋白（FN）在矽肺大鼠中的表达及定位，探讨Egr-1在矽肺发生发展中的作用。

方法

建立大鼠矽肺动物模型，采用免疫组织化学技术（SP法）及图像分析方法，分析矽肺大鼠各肺细胞中Egr-1、TGF-β(1)、FN的表达情况。

结果

在1～28天期间，矽肺组织中支气管上皮细胞、肺巨噬细胞、肺泡上皮细胞及间质细胞中Egr-1的表达（灰度值：118.58±5.65～168.52±5.67）高于对照组（灰度值：166.23±5.23～188.12±8.35），且表达主要在细胞核；这些细胞中TGF-β(1)的表达（灰度值：123.49±5.65～170.24±3.56）也高于对照组（166.53±6.25～198.56±4.53），且表达主要在细胞质。支气管上皮细胞、肺巨噬细胞及肺泡上皮细胞中FN的表达（灰度值：150.32±6.54～201.54±7.38）较低，而间质细胞中FN的表达（灰度值：121.43±5.65～167.55±6.35）高于对照组。在实验期间（1～28天），支气管上皮细胞、肺巨噬细胞、肺泡上皮细胞及间质细胞中TGF-β(1)和Egr-1表达水平的变化是同步的。二者相互相关（r = 0.61，P < 0.01），而FN的表达与Egr-1不相关，但与间质细胞中的TGF-β(1)相关（r = 0.46，P < 0.01）。