Luo Hai-bo, Zheng Shan-gen, Zhu Ping, Fu Ning
Department of Immunology, First Military Medical University, Guangzhou 510515, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Nov;20(6):682-5.
To screen mimotopes of E.coli lipopolysaccharide(LPS) 2630 from c7c phage display peptide library.
The LPS mimotopes were screened from c7c phage display peptide library by using affinity chromatograph-purified polyclonal antibody against E.coli LPS 2630(L2630), and the antigenicity of selected clones was identified by ELISA.
After 3 rounds of biopanning, 12 out of 20 phage clones were identified as positive clones which could bind to polyclonal anti-L2630 antibody, and 5 of these 12 clones could bind to polyclonal anti-S.typhi LPS 7261(L7261) antibody. The deduced amino acid sequence analysis showed that 8 of 12 clones had the conservative sequence: X-DGLL-XX or X-EDGLL-X.
The peptides displayed on these phage clones can mimic the epitopes of L2630, and 5 of these phage clones mimic the common epitopes of L2630 and L7261.
从c7c噬菌体展示肽库中筛选大肠杆菌脂多糖（LPS）2630的模拟表位。
用亲和层析纯化的抗大肠杆菌LPS 2630（L2630）多克隆抗体从c7c噬菌体展示肽库中筛选LPS模拟表位，并用酶联免疫吸附测定法（ELISA）鉴定所选克隆的抗原性。
经过3轮生物淘选，20个噬菌体克隆中有12个被鉴定为能与抗L2630多克隆抗体结合的阳性克隆，这12个克隆中的5个能与抗伤寒沙门菌LPS 7261（L7261）多克隆抗体结合。推导的氨基酸序列分析表明，12个克隆中的8个具有保守序列：X-DGLL-XX或X-EDGLL-X。
这些噬菌体克隆展示的肽可模拟L2630的表位，其中5个噬菌体克隆模拟L2630和L7261的共同表位。
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