Atg21p对于多形汉逊酵母中的巨自噬和微自噬至关重要。

Atg21p is essential for macropexophagy and microautophagy in the yeast Hansenula polymorpha.

作者信息

Leão-Helder Adriana N, Krikken Arjen M, Gellissen Gerd, van der Klei Ida J, Veenhuis Marten, Kiel Jan A K W

机构信息

Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.

出版信息

FEBS Lett. 2004 Nov 19;577(3):491-5. doi: 10.1016/j.febslet.2004.10.055.

DOI:10.1016/j.febslet.2004.10.055

PMID:15556634

Abstract

ATG genes are required for autophagy-related processes that transport proteins/organelles destined for proteolytic degradation to the vacuole. Here, we describe the identification and characterisation of the Hansenula polymorpha ATG21 gene. Its gene product Hp-Atg21p, fused to eGFP, had a dual location in the cytosol and in peri-vacuolar dots. We demonstrate that Hp-Atg21p is essential for two separate modes of peroxisome degradation, namely glucose-induced macropexophagy and nitrogen limitation-induced microautophagy. In atg21 cells subjected to macropexophagy conditions, sequestration of peroxisomes tagged for degradation is initiated but fails to complete.

摘要

自噬相关过程需要ATG基因，这些过程将注定要进行蛋白水解降解的蛋白质/细胞器转运到液泡中。在此，我们描述了多形汉逊酵母ATG21基因的鉴定和表征。其基因产物Hp-Atg21p与eGFP融合，在细胞质和液泡周围的点状结构中都有定位。我们证明，Hp-Atg21p对于过氧化物酶体降解的两种不同模式至关重要，即葡萄糖诱导的大自噬性过氧化物酶体降解和氮限制诱导的微自噬。在经历大自噬性过氧化物酶体降解条件的atg21细胞中，标记用于降解的过氧化物酶体的隔离开始，但未能完成。