Monastyrska Iryna, Kiel Jan A K W, Krikken Arjen M, Komduur Janet A, Veenhuis Marten, van der Klei Ida J
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.
Autophagy. 2005 Jul;1(2):92-100. doi: 10.4161/auto.1.2.1832. Epub 2005 Jul 10.
We have isolated the Hansenula polymorpha ATG25 gene, which is required for glucose-induced selective peroxisome degradation by macropexophagy. ATG25 represents a novel gene that encodes a 45 kDa coiled-coil protein. We show that this protein colocalizes with Atg11 on a small structure, which most likely represents the pre-autophagosomal structure (PAS). In cells of a constructed ATG25 deletion strain (atg25) peroxisomes are constitutively degraded by nonselective microautophagy, a process that in WT H. polymorpha is only observed at nitrogen limitation conditions. This suggests that nonselective microautophagy is deregulated in H. polymorpha atg25 cells.
我们分离出了多形汉逊酵母ATG25基因，该基因是通过巨自噬进行葡萄糖诱导的选择性过氧化物酶体降解所必需的。ATG25代表一个编码45 kDa卷曲螺旋蛋白的新基因。我们发现该蛋白与Atg11在一个小结构上共定位，这个小结构很可能代表自噬前体结构（PAS）。在构建的ATG25缺失菌株（atg25）的细胞中，过氧化物酶体通过非选择性微自噬持续降解，而在野生型多形汉逊酵母中，这个过程仅在氮限制条件下观察到。这表明在多形汉逊酵母atg25细胞中，非选择性微自噬失调。
