采用流动注射-鲁米诺化学发光检测法测定血清中的铁

Determination of iron in blood serum using flow injection with luminol chemiluminescence detection.

作者信息

Waseem A, Yaqoob M, Nabi A

机构信息

Department of Chemistry, University of Baluchistan, Quetta, Pakistan.

出版信息

Luminescence. 2004 Nov-Dec;19(6):333-8. doi: 10.1002/bio.787.

DOI:10.1002/bio.787

PMID:15558800

Abstract

A simple and rapid fl ow injection method is reported for the determination of iron in blood serum after acid digestion with HNO3 and HClO4, based on luminol CL detection in the absence of added oxidant. The detection limit (3 s) was 1.0 nmol/L with a sample throughput of 120/h. The calibration graph was linear over the range 0.001-1.0 micromol/L (r2 = 0.9974), with relative standard deviations (RSD) (n = 4) in the range 3.2-5%. The effect of interfering cations (Ca(II), Mg(II), Cu(II), Cd(II), Pb(II), Mn(II), Zn(II), Ni(II), Co(II) and Fe(III)) and anions (Cl-, SO4(2-), HCO3-, NO3-, NO2-) were studied using a luminol CL system for Fe(II) determination. The method was applied to normal blood serum and the results (1.32 +/- 0.08-1.74 +/- 0.05 mg/L) were compared with those from a spectrophotometric reference method (1.34 +/- 0.06-1.80 +/- 0.10 mg/L), which agree fairly well with the overall reference range in blood.

摘要

报道了一种简单快速的流动注射法，用于在硝酸和高氯酸酸消解血清后测定血清中的铁，该方法基于在不添加氧化剂的情况下鲁米诺化学发光检测。检测限（3s）为1.0 nmol/L，样品通量为每小时120个。校准曲线在0.001 - 1.0 μmol/L范围内呈线性（r2 = 0.9974），相对标准偏差（RSD）（n = 4）在3.2 - 5%范围内。使用鲁米诺化学发光系统研究了干扰阳离子（Ca(II)、Mg(II)、Cu(II)、Cd(II)、Pb(II)、Mn(II)、Zn(II)、Ni(II)、Co(II)和Fe(III)）和阴离子（Cl-、SO4(2-)、HCO3-、NO3-、NO2-）对铁（II）测定的影响。该方法应用于正常血清，其结果（1.32±0.08 - 1.74±0.05 mg/L）与分光光度参考方法的结果（1.34±0.06 - 1.80±0.10 mg/L）进行了比较，与血液中的总体参考范围相当吻合。