基于鲁米诺-铁氰化物反应的流动注射抑制化学发光法测定吲达帕胺
Flow-injection inhibition chemiluminescence determination of indapamide based on luminol-ferricyanide reaction.
作者信息
Wang Zhouping, Zhang Zhujun, Zhang Xiao, Fu Zhifeng
机构信息
Department of Chemistry, Analytical Science Institute, Southwest China Normal University, Chongqing 400715, China.
出版信息
J Pharm Biomed Anal. 2004 Apr 1;35(1):1-7. doi: 10.1016/S0731-7085(03)00642-3.
A novel and sensitive chemiluminescence (CL) method for the determination of indapamide coupled with flow-injection analysis (FIA) technique is developed in this paper. It is based on the inhibition effect of the studied drug on the chemiluminescence emission of luminol-potassium ferricyanide system. Under the optimum conditions, the decreased CL intensity is proportional with the concentration of indapamide in the range of 1 x 10(-8) to 1.0 x 10(-6) g ml(-1). The detection limit is 3.4 x 10(-9) g ml(-1) (3sigma). A complete analysis could be performed in 45 s including washing and sampling, giving a throughout of about 90 h(-1). The relative standard deviation (R.S.D.) for 11 parallel measurements of 1.0 x 10(-7) g ml(-1) indapamide is 3.0%. The proposed method has been applied for the determination of indapamide in its pharmaceutical formulations. The results obtained compared well with those by an official method. The possible inhibition mechanism of indapamide on luminol-potassium ferricyanide CL system was discussed briefly.
本文建立了一种结合流动注射分析（FIA）技术测定吲达帕胺的新型灵敏化学发光（CL）方法。该方法基于所研究药物对鲁米诺-铁氰化钾体系化学发光发射的抑制作用。在最佳条件下，化学发光强度的降低与吲达帕胺浓度在1×10⁻⁸至1.0×10⁻⁶ g ml⁻¹范围内成正比。检测限为3.4×10⁻⁹ g ml⁻¹（3σ）。包括清洗和进样在内，一次完整分析可在45秒内完成，进样频率约为90 h⁻¹。对1.0×10⁻⁷ g ml⁻¹吲达帕胺进行11次平行测定的相对标准偏差（R.S.D.）为3.0%。该方法已应用于吲达帕胺药物制剂的测定。所得结果与官方方法的结果吻合良好。简要讨论了吲达帕胺对鲁米诺-铁氰化钾化学发光体系可能的抑制机制。
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