Magae J, Hino A, Isono K, Nagai K
Department of Bioengineering, Tokyo Institute of Technology, Japan.
J Antibiot (Tokyo). 1992 Feb;45(2):246-51. doi: 10.7164/antibiotics.45.246.
Phorbol dibutyrate induced a nitroblue tetrazolium-reducing reaction in differentiated HL-60 cells, which was inhibited by protein kinase inhibitors such as staurosporine and H-7. ID50 of staurosporine and H-7 were 1.4 ng/ml and 0.19 mM, respectively. When tautomycin, an inhibitor of protein phosphatases, was added with the kinase inhibitors, the nitroblue tetrazolium-reducing reaction again appeared. ID50 of staurosporine was 510 ng/ml in the presence of tautomycin. Tautomycin itself weakly induced the reaction, which was inhibited by kinase inhibitors. Such a competitive effect between tautomycin and staurosporine was not observed in a cell-free system of protein kinase C. Okadaic acid had the same effect as tautomycin. The similar results were obtained when respiratory burst was quantitated by measuring H2O2 produced by canine peripheral neutrophils. The mechanism of competitive effect of tautomycin and staurosporine on respiratory burst is discussed.
佛波醇二丁酸酯在分化的HL-60细胞中诱导了硝基蓝四氮唑还原反应，该反应被星形孢菌素和H-7等蛋白激酶抑制剂所抑制。星形孢菌素和H-7的半数抑制浓度（ID50）分别为1.4 ng/ml和0.19 mM。当蛋白磷酸酶抑制剂陶霉菌素与激酶抑制剂一起添加时，硝基蓝四氮唑还原反应再次出现。在存在陶霉菌素的情况下，星形孢菌素的ID50为510 ng/ml。陶霉菌素本身可微弱地诱导该反应，该反应被激酶抑制剂所抑制。在蛋白激酶C的无细胞系统中未观察到陶霉菌素和星形孢菌素之间的这种竞争效应。冈田酸具有与陶霉菌素相同的作用。当通过测量犬外周血中性粒细胞产生的H2O2来定量呼吸爆发时，获得了相似的结果。本文讨论了陶霉菌素和星形孢菌素对呼吸爆发的竞争作用机制。
