在瑞斯托菌素A固定相上通过微高效液相色谱法对天然和非天然氨基酸衍生物进行手性分离。
Chiral separation of natural and unnatural amino acid derivatives by micro-HPLC on a Ristocetin A stationary phase.
作者信息
Piccinini Anna-Maria, Schmid Martin G, Pajpanova Tamara, Pancheva Svetlana, Grueva Ekatarina, Gübitz Gerald
机构信息
Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, Karl-Franzens University, Universitätsplatz 1, A-8010 Graz, Austria.
出版信息
J Biochem Biophys Methods. 2004 Oct 29;61(1-2):11-21. doi: 10.1016/j.jbbm.2004.02.004.
This paper deals with the chiral separation of Fmoc- and Z-derivatives of natural and unnatural sulfur containing amino acids by micro-HPLC. The separations were carried out in microbore columns packed with a new material based on Ristocetin A bonded to 3.5 microm silica gel. The columns were run in the normal phase, polar organic mode as well as in the reversed phase mode, whereby best results were obtained with the reversed-phase mode using mixtures of triethylamine acetate (TEAA) buffer and methanol as mobile phases.
本文研究了通过微径高效液相色谱法对手性天然和非天然含硫氨基酸的Fmoc和Z衍生物进行拆分。分离是在填充有基于瑞斯托菌素A键合到3.5微米硅胶的新型材料的微径柱中进行的。这些柱子在正相、极性有机模式以及反相模式下运行，其中使用三乙胺乙酸盐（TEAA）缓冲液和甲醇的混合物作为流动相的反相模式获得了最佳结果。
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