Sitton Alberto, Schmid Martin G, Gübitz Gerald, Aboul-Enein Hassan Y
Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, Karl-Franzens University, Univ. Pl.1, Graz 8010, Austria.
J Biochem Biophys Methods. 2004 Oct 29;61(1-2):119-24. doi: 10.1016/j.jbbm.2004.04.001.
The present study deals with the development of a method for the quantitative determination of lipoic acid in a dietary supplement preparation. A rapid capillary electrophoretic method is developed using UV detection at 208 nm. Although lipoic acid is only weakly UV-absorbing, at this wavelength it could be detected with sufficient sensitivity with an LOD and LOQ of 0.8 and 2.5 microg/ml, respectively. Analysis time was less than 9 min. The compound was extracted from tablets with a recovery of 98.3% and a precision of 2.8% RSD.
本研究致力于开发一种用于定量测定膳食补充剂制剂中硫辛酸的方法。采用在208 nm处进行紫外检测，开发了一种快速毛细管电泳方法。尽管硫辛酸的紫外吸收较弱，但在此波长下，其检测限和定量限分别为0.8和2.5μg/ml，能够以足够的灵敏度进行检测。分析时间不到9分钟。该化合物从片剂中提取，回收率为98.3%，精密度为2.8%相对标准偏差。
