Nicchia G P, Nico B, Camassa L M A, Mola M G, Loh N, Dermietzel R, Spray D C, Svelto M, Frigeri A
Department of General and Environmental Physiology and Centre of Excellence in Comparative Genomics (CEGBA), University of Bari, via Amendola 165/A, I-70126 Bari, Italy.
Neuroscience. 2004;129(4):935-45. doi: 10.1016/j.neuroscience.2004.07.055.
Aquaporin-4 (AQP4) is the major water channel expressed in brain perivascular astrocyte processes. Although the role of AQP4 in brain edema has been extensively investigated, little information exists regarding its functional role at the blood-brain barrier (BBB). The purpose of this work is to integrate previous and recent data regarding AQP4 expression during BBB formation and depending on BBB integrity, using several experimental models. Results from studies on the chick optic tectum, a well-established model of BBB development, and the effect of lipopolysaccharide on the BBB integrity and on perivascular AQP4 expression have been analyzed and discussed. Moreover, data on the BBB structure and AQP4 expression in murine models of Duchenne muscular dystrophy are reviewed. In particular, published results obtained from mdx(3cv) mice have been analyzed together with new data obtained from mdx mice in which all the dystrophin isoforms including DP71 are strongly reduced. Finally, the role of the endothelial component on AQP4 cellular expression and distribution has been investigated using rat primary astrocytes and brain capillary endothelial cell co-cultures as an in vitro model of BBB.
水通道蛋白4（AQP4）是脑周血管星形胶质细胞突起中表达的主要水通道。尽管AQP4在脑水肿中的作用已得到广泛研究，但关于其在血脑屏障（BBB）中的功能作用的信息却很少。这项工作的目的是利用几种实验模型，整合先前和最近关于BBB形成过程中以及取决于BBB完整性的AQP4表达的数据。对鸡视顶盖（一种成熟的BBB发育模型）的研究结果，以及脂多糖对BBB完整性和血管周围AQP4表达的影响进行了分析和讨论。此外，还综述了杜氏肌营养不良症小鼠模型中BBB结构和AQP4表达的数据。特别是，对从mdx(3cv)小鼠获得的已发表结果与从mdx小鼠获得的新数据进行了分析，在mdx小鼠中，包括DP71在内的所有肌营养不良蛋白异构体都大幅减少。最后，使用大鼠原代星形胶质细胞和脑毛细血管内皮细胞共培养作为BBB的体外模型，研究了内皮成分对AQP4细胞表达和分布的作用。
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