用于快速区分柠檬酸杆菌属细菌产生的胞外多糖中氨基葡萄糖和半乳糖胺的改良硼酸盐方法

Improved borate method for the rapid distinction of glucosamine and galactosamine in an exopolysaccharide produced by Citrobacter sp.

作者信息

Hia Hui Ching, Jang Jin-Ho, Ike Michihiko, Inoue Chiyo, Fujita Masanori, Yoshida Toshiomi

机构信息

Department of Environmental Engineering, Graduate School of Engineering, Osaka University, Suita city, Osaka, Japan.

出版信息

Biotechnol Lett. 2004 Aug;26(16):1307-11.

PMID:15562551

Abstract

An improved borate method for the quantitative distinction of glucosamine (GlcN) and galactosamine (GalN) in a mixture is presented which is based on the Elson-Morgan method with addition of sodium borate to differentiate colour formation by the two hexosamines. The r2 value and maximum deviation of the method based on calculations derived in this study were 0.9979 and 5.1 %, respectively. Using this method, the GlcN/GalN ratio in an exopolysaccharide (EPS) produced by Citrobacter sp. was found to change with time during the production process, with a maximum value at 9.8:1.

摘要

本文提出了一种改进的硼酸盐方法，用于定量区分混合物中的葡萄糖胺（GlcN）和半乳糖胺（GalN）。该方法基于埃尔森-摩根法，通过添加硼酸钠来区分两种己糖胺形成的颜色。根据本研究中的计算，该方法的r2值和最大偏差分别为0.9979和5.1%。使用该方法发现，柠檬酸杆菌属产生的胞外多糖（EPS）中的GlcN/GalN比值在生产过程中随时间变化，最大值为9.8:1。