柠檬酸杆菌属细菌产生的一种胞外多糖的酸水解及总己糖胺的定量测定

Acid hydrolysis and quantitative determination of total hexosamines of an exopolysaccharide produced by Citrobacter sp.

作者信息

Jang Jin-Ho, Hia Hui Ching, Ike Michihiko, Inoue Chiyo, Fujita Masanori, Yoshida Toshiomi

机构信息

Department of Environmental Engineering, Graduate School of Engineering, Japan.

出版信息

Biotechnol Lett. 2005 Jan;27(1):13-8. doi: 10.1007/s10529-004-6305-y.

DOI:10.1007/s10529-004-6305-y

PMID:15685413

Abstract

During the hydrolysis of an exopolysaccharide (EPS) produced by Citrobacter sp., the maximum liberation of hexosamine was obtained with 6 M HCl at 115 degrees C in an autoclave for 1 h. The glycosidic bond energy and degree of acetylation of the hexosamine in EPS were approximately 77 kJ mol(-1) and 61%, respectively. Thermal destruction of the hexosamines and the effect of salt on the hexosamine determination could be minimized under the optimized hydrolytic conditions. Using a modified Elson-Morgan method, maximum total hexosamine concentration was determined to be 3.2 g l(-1) (29% of crude EPS) after 96 h of fed-batch culture.

摘要

在柠檬酸杆菌属细菌产生的胞外多糖（EPS）水解过程中，使用6 M盐酸在115℃高压灭菌1小时可实现己糖胺的最大释放量。EPS中己糖胺的糖苷键能和乙酰化程度分别约为77 kJ mol⁻¹和61%。在优化的水解条件下，己糖胺的热破坏以及盐对己糖胺测定的影响可降至最低。采用改良的埃尔森 - 摩根法，在分批补料培养96小时后，测得总己糖胺的最大浓度为3.2 g l⁻¹（占粗EPS的29%）。