[通过在小沟中具有平行取向的聚酰胺来稳定含G x C的DNA双链体]

[Stabilization of G x C-containing DNA duplexes by polyamides with parallel orientation in the minor groove].

作者信息

Siniakov A N, Butorin A S, Héléne C, Riabinin V A

出版信息

Bioorg Khim. 2004 Sep-Oct;30(5):558-60.

Abstract

The polyamides based on 4-amino-1-methylpyrrol-2-carboxylic acid, 4-amino-1-methylimidazole-2-carboxylic acid, and beta-alanine that stabilize oligonucleotide duplexes consisting of G x C pairs through parallel packing in the minor groove were studied. The initial duplex TTGCGCp x GCGCAA melts at 28 degrees C; the TTGCGCp[NH(CH2)3COPyIm betaImNH(CH2)3NH(CH3)2][NH(CH2)3COIm betaImPyNH(CH2)3N(CH3)2] x GCGCAA duplex (bisphosphoramidate with parallel orientation of ligands, where Py, Im, and beta are the residues of 1-methyl-4-aminopyrrol-2-carboxylic and 1-methyl-4-aminoimidazole-2-carboxylic acids and beta-alanine, respectively), at 48 degrees C; and the TTGCGCp[NH(CH2)3COIm betaImPyNH(CH2)3COIm betaImPyNH(CH2)3N(CH3)2] x GCGCAA duplex (a hairpin structure with antiparallel orientation), at 56 degrees C. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 5; see also http: // www.maik.ru.

摘要

对基于4-氨基-1-甲基吡咯-2-羧酸、4-氨基-1-甲基咪唑-2-羧酸和β-丙氨酸的聚酰胺进行了研究，这些聚酰胺通过在小沟中平行堆积来稳定由G×C碱基对组成的寡核苷酸双链体。初始双链体TTGCGCp×GCGCAA在28℃时解链；双链体TTGCGCp[NH(CH2)3COPyImβImNH(CH2)3NH(CH3)2][NH(CH2)3COImβImPyNH(CH2)3N(CH3)2]×GCGCAA（配体平行排列的双磷酰胺酯，其中Py、Im和β分别是1-甲基-4-氨基吡咯-2-羧酸、1-甲基-4-氨基咪唑-2-羧酸和β-丙氨酸的残基）在48℃时解链；而双链体TTGCGCp[NH(CH2)3COImβImPyNH(CH2)3COImβImPyNH(CH2)3N(CH3)2]×GCGCAA（反平行排列的发夹结构）在56℃时解链。论文英文版：《俄罗斯生物有机化学杂志》，2004年，第30卷，第5期；另见http://www.maik.ru。