Hirano Shigeru, Bless Diane M, del Río Alejandro Muñoz, Connor Nadine P, Ford Charles N
From the Department of Bronchoesophagology, Kyoto National Hospital, Kyoto, Japan.
Laryngoscope. 2004 Dec;114(12):2161-7. doi: 10.1097/01.mlg.0000149450.37640.db.
OBJECTIVES/HYPOTHESIS: It has been reported that in aged vocal folds, dense collagen deposition takes place and hyaluronic acid decreases in the lamina propria, which are thought to contribute to the vocal problems occurring with age (presbyphonia). To restore aged vocal folds to their younger state, it seems crucial to address these age-related lamina propria changes. Intervention that might increase hyaluronic acid and decrease collagen would appear to be a potentially useful approach. The present study examined the effects of growth factors on aged fibroblasts in terms of the production of hyaluronic acid and collagen type I.
In vitro study using animal model.
Fibroblasts were harvested from young and aged rat vocal folds and cultured with or without hepatocyte growth factor and/or basic fibroblast growth factor at different concentrations. Subsequently, the production of hyaluronic acid and collagen type I was examined in the supernatant culture media using enzyme-linked immunosorbent assay.
Aged fibroblasts produced less hyaluronic acid than younger fibroblasts. When aged and young fibroblasts were cultured with basic fibroblast growth factor, hyaluronic acid production increased and collagen type I production decreased regardless of the concentration, whereas the effects of hepatocyte growth factor appeared to vary with concentration. The basic fibroblast growth factor also was associated with stimulation of growth of aged fibroblasts.
The present results suggest that growth factors, especially basic fibroblast growth factor, may have therapeutic potential in restoration of aged vocal fold.
目的/假设：据报道，在老年声带中，固有层会发生致密的胶原蛋白沉积，透明质酸减少，这些变化被认为是导致与年龄相关的嗓音问题（老年嗓音障碍）的原因。为了使老年声带恢复到年轻时的状态，解决这些与年龄相关的固有层变化似乎至关重要。增加透明质酸并减少胶原蛋白的干预措施似乎是一种潜在有用的方法。本研究从透明质酸和I型胶原蛋白的产生方面，研究了生长因子对老年成纤维细胞的影响。
使用动物模型的体外研究。
从年轻和老年大鼠声带中获取成纤维细胞，并在不同浓度的肝细胞生长因子和/或碱性成纤维细胞生长因子存在或不存在的情况下进行培养。随后，使用酶联免疫吸附测定法检测上清液培养基中透明质酸和I型胶原蛋白的产生。
老年成纤维细胞产生的透明质酸比年轻成纤维细胞少。当老年和年轻成纤维细胞与碱性成纤维细胞生长因子一起培养时，无论浓度如何，透明质酸的产生都会增加，I型胶原蛋白的产生会减少，而肝细胞生长因子的作用似乎随浓度而变化。碱性成纤维细胞生长因子也与老年成纤维细胞的生长刺激有关。
目前的结果表明，生长因子，尤其是碱性成纤维细胞生长因子，可能在恢复老年声带方面具有治疗潜力。
