Fu Geng-Feng, Li Xi, Hou Ya-Yi, Fan Yan-Rong, Liu Wen-Hua, Xu Gen-Xing
Medical School, Nanjing University, Nanjing, China.
Cancer Gene Ther. 2005 Feb;12(2):133-40. doi: 10.1038/sj.cgt.7700758.
To overcome difficulties that hampered widespread application of a specific delivery system in cancer gene therapy and to inhibit the growth of solid liver cancer, we utilized a strain of Bifidobacterium longum as a delivery system to transport an endostatin gene that can inhibit growth of tumor. The B. longum strain with the endostatin gene (B. longum-En) was taken orally by tumor-bearing nude mice through drencher preparation. The results showed that B. longum-En could strongly inhibit the growth of solid liver tumor in nude mice and prolong the survival time of tumor-bearing nude mice. Furthermore, tumor growth was inhibited more efficiently when the B. longum-En treatment included selenium. Enriching the B. longum-En treatment with selenium improves the activity of NK and T cells and stimulates the activity of IL-2 and TNF-alpha in BALB/c mice. These results suggest that B. longum may be a highly specific and efficient vector for transporting anticancer genes in cancer gene therapy.
为克服阻碍特定递送系统在癌症基因治疗中广泛应用的困难并抑制实体肝癌的生长，我们利用一株长双歧杆菌作为递送系统来转运可抑制肿瘤生长的内皮抑素基因。携带内皮抑素基因的长双歧杆菌菌株（长双歧杆菌-内皮抑素）通过灌胃制剂经荷瘤裸鼠口服。结果表明，长双歧杆菌-内皮抑素能强烈抑制裸鼠体内实体肝肿瘤的生长并延长荷瘤裸鼠的存活时间。此外，当长双歧杆菌-内皮抑素治疗中加入硒时，肿瘤生长受到更有效的抑制。用硒富集长双歧杆菌-内皮抑素治疗可提高BALB/c小鼠中NK和T细胞的活性，并刺激IL-2和TNF-α的活性。这些结果表明，长双歧杆菌可能是癌症基因治疗中转运抗癌基因的高度特异性和高效载体。
