Kodama Shuji, Yamamoto Atsushi, Iio Reiko, Sakamoto Keiji, Matsunaga Akinobu, Hayakawa Kazuichi
Toyama Institute of Health, 17-1 Nakataikoyama, Kosugi-machi, Toyama 939-0363, Japan.
Analyst. 2004 Dec;129(12):1238-42. doi: 10.1039/b406875k. Epub 2004 Oct 21.
Native DL-pantothenic acid, having a 1,3-diol structure, was chirally resolved by ligand exchange capillary electrophoresis using (S)-3-amino-1,2-propanediol as a chiral selector and the borate anion as a central ion. The optimum conditions for both high resolution and short migration time of DL-pantothenic acid were found to be 200 mM (S)-3-amino-1,2-propanediol and 200 mM borate buffer (pH 9.2) containing 15% methanol with an applied voltage of +25 kV at 20 degrees C, using direct detection at 200 nm. With this system, the resolution (Rs) of racemic pantothenic acid was approximately 1.7. When (S)-1,2-propanediol, (S)-1,2,3-propanetriol, (S)-1,3-butanediol or (S)-1-amino-2-propanol were used as chiral ligand instead of (S)-3-amino-1,2-propanediol, DL-pantothenic acid was not enantioseparated. When borate was replaced with Tris or butylborate, no chiral separation was achieved. Therefore, the ionic interaction between the amino and carboxyl groups of the ternary complex may play an important role in the enantioseparation of DL-pantothenic acid by the proposed CE system.
具有1,3 - 二醇结构的天然DL - 泛酸，通过配体交换毛细管电泳进行手性拆分，使用(S) - 3 - 氨基 - 1,2 - 丙二醇作为手性选择剂，硼酸根阴离子作为中心离子。发现DL - 泛酸实现高分辨率和短迁移时间的最佳条件为：200 mM (S) - 3 - 氨基 - 1,2 - 丙二醇和200 mM硼酸盐缓冲液（pH 9.2），含有15%甲醇，在20℃下施加+25 kV的电压，并在200 nm处进行直接检测。在该系统中，外消旋泛酸的分辨率（Rs）约为1.7。当使用(S) - 1,2 - 丙二醇、(S) - 1,2,3 - 丙三醇、(S) - 1,3 - 丁二醇或(S) - 1 - 氨基 - 2 - 丙醇代替(S) - 3 - 氨基 - 1,2 - 丙二醇作为手性配体时，DL - 泛酸未实现对映体分离。当用Tris或硼酸丁酯代替硼酸盐时，未实现手性分离。因此，三元配合物的氨基和羧基之间的离子相互作用可能在该毛细管电泳系统对DL - 泛酸的对映体分离中起重要作用。
