Díaz Gema, Cañas Benito, Vazquez Jesús, Nombela César, Arroyo Javier
Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040, Madrid, Spain.
Immunogenetics. 2005 Jan;56(10):754-9. doi: 10.1007/s00251-004-0735-5. Epub 2004 Nov 24.
Although natural peptide ligands of HLA-DR and HLA-DQ molecules have been extensively studied, information about peptides naturally bound to HLA-DP is limited. Here we describe HLA-DP2 peptide ligands corresponding to 24 different source proteins that were identified by peptide pool elution and mass spectrometry sequencing from HLA-DP2 molecules expressed on EBV-LCLs. Sequencing analysis led to the identification of both promiscuous and allele-specific peptides. Moreover, the alignment of the natural ligands for HLA-DP2 described here, combined with previous results from our group and others concerning HLA-DP2 antigen presentation and HLA-DP molecular modelling, provide a better understanding of HLA-DP2 peptide-binding motifs.
尽管HLA-DR和HLA-DQ分子的天然肽配体已得到广泛研究，但关于天然结合到HLA-DP的肽的信息却很有限。在此，我们描述了与24种不同来源蛋白质相对应的HLA-DP2肽配体，这些配体是通过肽库洗脱和质谱测序从EBV-LCLs上表达的HLA-DP2分子中鉴定出来的。测序分析导致了混杂肽和等位基因特异性肽的鉴定。此外，本文所述的HLA-DP2天然配体的比对，结合我们小组及其他小组先前关于HLA-DP2抗原呈递和HLA-DP分子建模的结果，能让我们更好地理解HLA-DP2肽结合基序。
