Lwamba Humphrey C M, Alvarez Rene, Wise Mark G, Yu Qingzhong, Halvorson David, Njenga M Kariuki, Seal Bruce S
Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA.
Virus Res. 2005 Jan;107(1):83-92. doi: 10.1016/j.virusres.2004.07.002.
We determined the nucleotide (nt) sequence of the small hydrophobic (SH), attachment glycoprotein (G), and RNA polymerase (L) genes, plus the leader and trailer regions of the Colorado strain of Avian metapneumovirus subtype C (aMPV/C) in order to complete the genome sequencing. The complete genome comprised of 13,134 nucleotides, with a 40 nt leader at its 3' end and a 45 nt trailer at its 5' end. The aMPV/C L gene was the largest with 6173 nt and consisting of a single open reading frame encoding a 2005 amino acids (aa) protein. Comparison of the aMPV/C SH, G, and L nt and predicted aa sequences with those of Human metapneumoviruses (hMPV) revealed higher nt and aa sequence identities than the sequence identities between the aMPV subtypes A, B, C, and D, supporting earlier finding that aMPV/C was closer evolutionary to hMPV than the other aMPV subtypes.
为完成基因组测序，我们测定了禽偏肺病毒C亚型（aMPV/C）科罗拉多株的小疏水（SH）基因、附着糖蛋白（G）基因和RNA聚合酶（L）基因的核苷酸（nt）序列，以及前导区和尾随区。完整基因组由13,134个核苷酸组成，其3'端有一个40 nt的前导序列，5'端有一个45 nt的尾随序列。aMPV/C的L基因最大，有6173 nt，由一个单一的开放阅读框组成，编码一个2005个氨基酸（aa）的蛋白质。将aMPV/C的SH、G和L的nt序列及预测的aa序列与人偏肺病毒（hMPV）的相应序列进行比较，结果显示其nt和aa序列同一性高于aMPV A、B、C和D亚型之间的序列同一性，这支持了早期的发现，即aMPV/C在进化上比其他aMPV亚型更接近hMPV。
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