Wetzel T, Dietzgen R G, Dale J L
Queensland University of Technology, Centre for Molecular Biotechnology, School of Life Sciences, Gardens Point, Brisbane, Australia.
Virology. 1994 May 1;200(2):401-12. doi: 10.1006/viro.1994.1204.
We have mapped the genome of lettuce necrotic yellows virus (LNYV), the type member of the genus cytorhabdovirus of the family Rhabdoviridae. We have cloned and sequenced all intergenic regions and the 3' leader and 5' trailer of the negative-sense, single-stranded RNA genome of LNYV. The LNYV genome appears to contain six genes, the five expected genes coding for the virion proteins, and a sixth gene of unknown function, as for sonchus yellow net virus (SYNV), a member of the genus nucleorhabdovirus. The proposed LNYV genomic map is 3'-N-4a-4b-M-G-L-5', where N is the nucleocapsid protein gene; 4a and 4b are two genes, one of which codes for the proposed phosphoprotein P and the other for a putative protein of unknown function; M is the proposed matrix protein gene; G is the proposed glycoprotein gene; and L is the proposed transcriptase gene. The different LNYV intergenic regions have highly conserved consensus sequences, which could be divided into three components: the sequences corresponding to the 3' end of the mRNAs, intergenic sequences of variable length, and the sequences corresponding to the 5' end of the mRNAs. A leader sequence of 84 nucleotides (nt) at the 3' end of the LNYV genomic RNA preceeded the N gene. A trailer sequence of 187 nt at the 5' end of the genomic RNA followed the L gene. A comparison between LNYV leader and trailer sequences revealed complementary 3' and 5' ends, which could give rise to a putative "panhandle" structure with a two bases overhang in the leader sequence. We have compared these sequences to the corresponding sequences of SYNV as well as to vesicular stomatitis virus (VSV) and rabies virus (RV), the type members of the vesiculovirus and lyssavirus genera, respectively, of animal rhabdoviruses. Homologies were found in the intergenic regions between LNYV, SYNV, VSV, and RV, at the 3' ends of the mRNAs. LNYV intergenic sequences were of variable lengths, as were those found in RV. The consensus sequences found at the 5' ends of LNYV mRNAs differed from the highly conserved consensus transcription start sequence UUGU/A found in SYNV, VSV, and RV. Conserved sequences were also found in the first 30 nt of the leader and the last 30 nt of the trailer, between LNYV, SYNV, VSV, and RV.
我们已绘制出莴苣坏死黄化病毒（LNYV）的基因组图谱，该病毒是弹状病毒科细胞质弹状病毒属的典型成员。我们已克隆并测序了LNYV负链单链RNA基因组的所有基因间隔区以及3'前导序列和5'尾随序列。LNYV基因组似乎包含六个基因，五个是预期的编码病毒粒子蛋白的基因，还有一个功能未知的第六个基因，就像核质弹状病毒属的成员苣买菜黄网病毒（SYNV）一样。提议的LNYV基因组图谱为3'-N-4a-4b-M-G-L-5'，其中N是核衣壳蛋白基因；4a和4b是两个基因，其中一个编码提议的磷蛋白P，另一个编码功能未知的假定蛋白；M是提议的基质蛋白基因；G是提议的糖蛋白基因；L是提议的转录酶基因。不同的LNYV基因间隔区具有高度保守的共有序列，可分为三个部分：与mRNA 3'端对应的序列、可变长度的基因间隔序列以及与mRNA 5'端对应的序列。LNYV基因组RNA 3'端有一个84个核苷酸（nt）的前导序列位于N基因之前。基因组RNA 5'端有一个187 nt的尾随序列位于L基因之后。LNYV前导序列和尾随序列的比较揭示了互补的3'和5'末端，这可能会形成一个在其前导序列中有两个碱基突出的假定“锅柄”结构。我们已将这些序列与SYNV的相应序列以及水泡性口炎病毒（VSV）和狂犬病病毒（RV）的相应序列进行了比较，VSV和RV分别是动物弹状病毒中水泡病毒属和狂犬病病毒属的典型成员。在LNYV、SYNV、VSV和RV之间的基因间隔区以及mRNA的3'端发现了同源性。LNYV的基因间隔序列长度可变，RV中的情况也是如此。在LNYV mRNA 5'端发现的共有序列与在SYNV、VSV和RV中发现的高度保守的共有转录起始序列UUGU/A不同。在LNYV、SYNV、VSV和RV之间，在前导序列的前30 nt和尾随序列的后30 nt中也发现了保守序列。
