Berg Einar S, Størvold Gunnar, Anestad Gabriel, Moi Harald, Skaug Kjell
SMAB, Division for Infectious Disease Control, Norwegian Institute of Public Health, PO Box 4404 Nydalen, N-0403 Oslo, Norway.
J Microbiol Methods. 2005 Jan;60(1):125-9. doi: 10.1016/j.mimet.2004.09.002.
Four possibly false-negative samples were detected when 514 male urine specimens were tested in the Amplicor Chlamydia trachomatis assay. In three of the four samples, the inhibition could be reduced by removal of urine supernatant. Under partially inhibitory conditions, after spiking with 50 C. trachomatis elementary bodies/ml specimen, a selective inhibition of the C. trachomatis target amplification and a preferential internal control amplification was observed. We conclude that a positive internal control signal might be misleading in inhibitory specimens with low amount of C. trachomatis.
在用Amplicor沙眼衣原体检测法检测514份男性尿液标本时，检测到4份可能为假阴性的样本。在这4份样本中的3份中，通过去除尿液上清液可降低抑制作用。在部分抑制条件下，向每份标本中加入50个沙眼衣原体原体后，观察到沙眼衣原体靶标扩增受到选择性抑制，而内部对照优先扩增。我们得出结论，在沙眼衣原体数量较少的抑制性标本中，阳性内部对照信号可能会产生误导。
