[血清蛋白质组学中去除白蛋白和IgG方法的建立与评价]
[Establishment and evaluation of the method for removing albumin and IgG in serum proteomics].
作者信息
Li Xue-hua, Li Xin, Xu Shou-jun, Ding Yan-qing
机构信息
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2004 Nov;24(11):1233-5.
OBJECTIVE
To establish and evaluate the method for removing albumin and IgG in serum proteomics.
METHODS
Elimination of serum albumin and IgG was achieved by filtering with Micro Bio-Spin column and the efficiency of purification evaluated by two-dimensional polyacrylamide gel electrophoresis (2-DE).
RESULTS
The method for removing serum albumin and IgG was established, which enabled visualization of low-abundance proteins better in 2-DE map.
CONCLUSION
The established method for purifying serum proteins can be effectively applied in serum proteomic study of diseases.
目的
建立并评估血清蛋白质组学中去除白蛋白和IgG的方法。
方法
通过使用微生物旋转柱过滤实现血清白蛋白和IgG的去除，并通过二维聚丙烯酰胺凝胶电泳（2-DE）评估纯化效率。
结果
建立了去除血清白蛋白和IgG的方法，该方法能在2-DE图谱中更好地显示低丰度蛋白质。
结论
所建立的血清蛋白质纯化方法可有效应用于疾病的血清蛋白质组学研究。
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