Liu Peng, Huang Sheng-ping, Qi Song-tao, Zhang Yong-ming
Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2004 Nov;24(11):1242-4.
To evaluate the safety and efficacy of a dural graft prepared using porcine membrane in duraplasty.
Eighteen New Zealand rabbits were randomly divided into groups A (n=4), B (n=4), C (n=5), and D (n=5) sacrificed 3, 14, 30 and 90 d after duraplasty, respectively. Each animal underwent bilateral parietal craniectomy behind the coronal suture and beside the midline to expose the dura, which was cut on the right side and substituted with the dural graft. The exposed dura on the left was kept intact as control. The rabbits were observed for WBC counts before the operation and before sacrifice by transcardiac formalin perfusion, respectively. The meninges and brain tissues were histologically examined after sacrifice.
The WBC count varied little after the operation (P>0.05). Microscopic examination demonstrated tissue repair on both the implantation side and control side, without graft adhesion to the cortical surface. In group A, a large number of leukocytes were seen gathering on the lateral dura, suggesting acute tissue repair. In group B, endothelial cells covering the inner surface of the graft could be seen. Fibroblasts and fibrocytes were seen in the grafts between collagen fibers in group C, and in group D, fibroblasts and fibrocytes increased between the collagen fibers and the suture healed.
The dura graft is safe and applicable for dural defect repair.
评估使用猪脑膜制备的硬脑膜移植物在硬脑膜成形术中的安全性和有效性。
18只新西兰兔随机分为A组（n = 4）、B组（n = 4）、C组（n = 5）和D组（n = 5），分别在硬脑膜成形术后3天、14天、30天和90天处死。每只动物在冠状缝后方和中线旁进行双侧顶骨开颅术以暴露硬脑膜，右侧硬脑膜切开并用硬脑膜移植物替代。左侧暴露的硬脑膜保持完整作为对照。分别在手术前和处死前经心脏福尔马林灌注观察兔的白细胞计数。处死动物后对脑膜和脑组织进行组织学检查。
术后白细胞计数变化不大（P>0.05）。显微镜检查显示植入侧和对照侧均有组织修复，移植物未与皮质表面粘连。A组可见大量白细胞聚集在外侧硬脑膜上，提示急性组织修复。B组可见覆盖在移植物内表面的内皮细胞。C组在胶原纤维之间的移植物中可见成纤维细胞和纤维细胞，D组胶原纤维之间的成纤维细胞和纤维细胞增多且缝线愈合。
硬脑膜移植物用于硬脑膜缺损修复是安全可行的。
